Lactose intolerance PCR test (LCT 13910C>T)

The patient friendly solution to lactose intolerance testing

The Lactose intolerance is usually tested with a drink containing lactose. After two hours a blood sample will be taken to measure how much glucose is in the blood. If the blood glucose level does not rise, this means that the body is not digesting or absorbing lactose. This way of analyzing the lactose tolerance is very patient unfriendly.

A better solution would be to just take blood sample and run a molecular test. ViennaLab offers a LCT RealFast Assay. This PCR test tests on the LCT 13910C>T mutation.

Unique features of the RealFast Assays

The RealFast Assays from ViennaLab are a great product line. All RealFast assays can be combined in the same thermocylcer. This because the thermocycling program is identical for all RealFast Assays. this way the Lactose intolerance test can be combined with tests like HFE, FV and FII(PTH).

HLA-B27 PCR Test

HLA-B27 is a genetic risk factor for seronegative spondyloarthropathies, such as ankylosing spondylitis, reactive arthritis, juvenile rheumatoid arthritis and anterior uveitis.

The HLA-B27 PCR test from Viennalab is known for its high quality but especially for its exceptional speed. The very short PCR program ensures that the results are generated quickly and accurately. All Viennalab PCR tests can also be carried out with the same protocol. This means you do not have to save up your samples from various genetic analysis.  With the RealFast assays you can combine analysis like Haemachromatosis, Factor V leiden, PTH & HLA B27 in one PCR run.

Furthermore, the HLA-B27 PCR test can be combined with a verified fast and low priced DNA isolation kit, which means that the total costs can be kept interesting. More…

 

A new PCR-system to run a quick qPCR in 15 minutes?

quick qPCRYes! And it is coming to the BeNeLux!

The PCRmax Exo 48 utilises a patented block and an optical technologie to deliver extreme speed, sensitivity and data quality. Optimised protocols will run a quick qPCR in as fast as 15 minutes. This all while using standard plasticware and consumables.

But why “Eco”

A unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5µl is validated, cutting reagent costs in half and making more efficient use of expensive and hard to acquire template DNA samples. More…

Would you like to see how this quick qPCR system works?

Here is a video on the PCRmax ECO48 Real-time qPCR system.

Request a Free PCRmax Demo

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16S-23S Comparison SelectNa Plus, Qia-amp and Purelink

The implementation of 16S-23S Next Generation Sequencing (NGS) for the detection of pathogens in clinical samples is an emerging area in clinical diagnostics. Certe and the UMCG have developed a novel, culture independent, method that detects and identifies bacterial species directly from clinical samples by targeted NGS of the 16S-23S rDNA region.

A barrier is the ratio of eukaryote to pathogen DNA isolated from samples with a low pathogen load. DNA extraction methods that feature selective lysis of eukaryote cells and subsequent degradation of the released eukaryote DNA followed by isolation of purified microbial DNA could improve the sensitivity of 16S-23S rDNA NGS.

We compared the MolYsis SelectNA plus DNA extraction kit (Molzym) and QIAamp DNA Microbiome DNA extraction kit (Qiagen), both featuring eukaryote DNA depletion, with the Purelink Genomic DNA mini kit (Invitrogen) which extracts total DNA, for sensitivity and depletion of eukaryote DNA using 16S-23S rDNA NGS.

Please click below for the full article.

Comparison_MolYsis_SelectNA Plus

 

Comprehensive CE-IVD CYP2D6 assay

The cytochrome P450 2D6 (CYP2D6 assay) is an important liver enzyme involved in the metabolism of up to 25% of clinically used drugs (e.g. tamoxifen, opiates, anti-depressives or anti-psychotics). The CYP2D6 gene is highly polymorphic with over 100 allelic variants and numerous sub-variants described in the Human Cytochrom P450 Database (www.cypalleles.ki.se).

Request a FREE CYP2D6 kit!

While the most frequent allelic variations are caused by single nucleotide polymorphisms (SNPs) and small insertions or deletions, highly homologous regions in the CYP2D6 gene locus facilitate unequal cross-over leading to large deletions, duplications and gene conversions. Most of these alterations result in a change of the CYP2D6 enzyme activity. Guidelines recommend to determine the CYP2D6 genotype of patients prior to medication in order to be able to estimate the metabolic activity of the enzyme, and in consequence the clinical efficacy or even the applicability of defined drugs.

Please click below for the full article.

Comprehensive analysis of CYP2D6 variants and copy numbers using reverse-hybridization and real-time PCR based assays

Rapid detection Candida albicans and fungal pathogens in blood

Arcis BiotechnologyCandida albicans species are common human commensals that can cause disseminated infection.
In fact, Candidaalbicans (the major cause of invasive candidiasis), has become one of the pathogens most frequently isolated from the blood of postoperative and immunocompromised patients during the last decade.

However, diagnosis remains difficult and tests have been developed to detect circulating Candida antigens for rapid diagnosis of disseminated candidiasis, but no PCR-based test has set the standard for molecular detection yet. In this work, a rapid system for blood processing is described that allows extraction and stabilisation of all fungal genomic targets.

This novel technology has produced a proof of concept by teaming up with a new specific and sensitive qPCR test for the timely and accurate diagnosis Candida and concomitant fungal infections. A finer analysis within a small cohort of clinical specimens has allowed to set a basic LOD on par with blood culture, requiring a total time of 3 hours to complete the entire process (extraction and detection).

Click below for the full article.

A novel processing technology for simple and rapid detection of Candida albicansand fungal pathogens in blood by qPCR

Request a free DNA Pathogen extraction kit


Full DNA/RNA Isolation in 3 minutes

DNA RNA isolation

The Arcis DNA/RNA Isolation in 3 minutes Pathogen Kit is a ready to use kit comprising only two pre-filled reagent tubes enabling the extraction of nucleic acids from a variety of biological samples.  DNA RNA isolation

No centrifuge steps | No heating | High yield | Only 3 minutes | Ideal for transportation extracted DNA | Better than all other alternatives!

In 3 minutes, with no prior sample preparation, the Arcis Pathogen Kit allows you to go from bacterial samples to downstream nucleic acid investigations without the need for isolation or purification. The Arcis Pathogen Kit is an IVD sample prep system for the release of bacterial nucleic acids from bacteria grown on common microbiological growth substrates.  The material released is suitable for use in molecular diagnostic investigations such as PCR.

The Arcis DNA Pathogen Kit is intended for in vitro diagnostic use.  The Arcis Pathogen Kit can also be used in research (non IVD) to release DNA and RNA from pathogens in a variety od biological sample types including blood, swabs and saliva.

Please click  here for more information.

Request a free DNA Pathogen extraction kit


Complimentary Products: PCRmax, fast qPCR

The new PCRmax Eco48 real time PCR instrument is one of the fastest PCRs currently available on the market. In combination with the superfast DNA/RNA extraction kits from Arcis, an exceptional turnaround time is achieved.


 

ViennaLab Newsletter

ViennaLab is now releasing the first multiplexseries of multiplex (mpx) RealFast™ Assays. The ViennaLab multiplex RealFast™ Assays allow to genotype two loci simultaneously and thus save costs and sample material.

The following multiplex RealFast™ Assays will become available as of 1st week of May 2018:

The CYP2C9 mpx RealFast™ Assay is designed for the simultaneous detection of the c.430C>T (CYP2C9*2) and c.1075A>C (CYP2C9*3) polymorphisms in the human CYP2C9 gene. Both variants of the CYP2C9 enzyme, *2 and *3, exhibit decreased function leading to poor metabolism (PM) phenotypes for various drugs. Patients with low enzyme activity are at risk of adverse drug reactions or therapeutic failure, particularly for CYP2C9 substrates with a narrow therapeutic window, such as S-warfarin or phenytoin. The kit is designed to identify patients carrying one or two variants of the CYP2C9 *2 or *3 variants.

The Alpha-1 antitrypsin (AAT) mpx RealFast™ Assay is designed for the simultaneous detection of the protease inhibitor (PI) variants *S and *Z of the SERPINA1 gene. These are the most frequent alleles associated with alpha-1 antitrypsin deficiency. The kit is intended for use as a diagnostic tool to identify alpha-1 antitrypsin deficient patients carrying a PI*S or PI*Z allele.


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3 Minute DNA/RNA blood extraction kit

Arcis Biotechnology Arcis DNA/RNA Blood Extraction Kit is a ready to use kit comprising two pre-filled reagent tubes enabling extraction of nucleic acids from blood samples prior to a flexible range of downstream assays. 

No centrifuge steps | No heating | High yield | Only 3 minutes | Ideal for transportation extracted DNA | Better than all other alternatives!

DNA/RNA Isolation in 3 minutes, with no prior sample preparation. The kit allows you to go from blood to downstream nucleic acid investigations without the need for additional isolation or purification.

The Arcis Blood Kit will release nucleic acids from fresh, dried or frozen whole blood samples. The kit is also suitable for specimens that have been stored in EDTA and heparin containing solutions. The nucleic acids released are ready for use in PCR or other molecular applications.

Please click here for more information.

Request a free DNA Blood extraction kit


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Sample Prep Kit for any sample

Arcis BiotechnologyDNA/RNA general extraction kit

No centrifuge steps | No heating | High yield | Only 3 minutes | No inhibition  | 

The new Sample Prep Kit from Arcis is an outstanding ready to use DNA/RNA general extraction kit comprising two reagents tubes enabling flexible processing of a variety of sample types and sample volumes including whole blood, saliva, bacteria, plant or animal cells. The fast and convenient Arcis DNA Sample Prep kit allows you to go from cells to downstream nucleic acid investigations without the need for isolation or purification.

Request a free Sample Prep extraction kit

The product can be used on virtually any DNA or RNA containing sample. The nucleic acid released has been successfully applied to molecular biology techniques including PCR, RT-qPCR and sequencing.

The ARCIS Sample Prep Kit accelerates pre-analytical processing in the following targets:

  • Human Samples: including whole blood (fresh/frozen, with and without heparin or EDTA), saliva, plasma, urine and buccal swabs
  • Microbiological samples: including bacteria (E.coli, S.aureus, P.aeruginosa, K. pneumoniae), viruses(HBV/HCV) and parasites (plasmodium)

Please click  here for more information.


Complimentary Products: PCRmax, fast qPCR

The new PCRmax Eco48 real time PCR instrument is one of the fastest PCRs currently available on the market. In combination with the superfast DNA/RNA extraction kits from Arcis, an exceptional turnaround time is achieved.