RS Virus test

The Respiratory Syncytial (RS) virus is hightly contagious and can cause respiratory tract infections. This virus infects mostly young children. Symptoms of the RS virus are usually mild, but in some cases it can cause bronchiolitis leading to severe respiratory illness. Because this virus is very contagious by aerosols, rapid diagnosis can disturb further spreading.

Capilia RSV Neo provides a quick and accurate detection of RS virus antigens. The test is based on an immunochromatography assay using monoclonal antibodies that recognise RS virus antigenes. Capilia RSV Neo is usable for nasal swabs or aspirate samples. The samples are easily prepared for the RS virus antigen detection. Results are visible after 3 to 5 minutes. Read more…

New Viennalab tests for Carbamazepine hypersensitivity

ViennaLab is now releasing two real-time PCR based assays (RealFast Assay) for genotyping HLA-A*31:01 and HLA-B*15:02 in patients with an indication for carbamazepine (CBZ) treatment.

Both HLA alleles are associated with carbamazepine-induced hypersensitivity reactions in Europeans and in many Asian populations. According to the Clinical Pharmacogenetics Implementation Consortium (CPIC) guidlines, patients positive for HLA-A*31:01 or HLA-B*15:02 should not be treated with carbamazepine to reduce the risk of severe cutaneous adverse reactions.

All new ViennaLab HLA RealFast Assays (HLA-A3101, HLA-B1502, HLA-B27, HLA-B5701) will include a new separate negative control for data analysis.

To view the product brochure, click here.




Tauns Capilia TB-Neo test

Tuberculosis (TB) is a chronic disease caused by infection with Mycobacterium tuberculosis. The annual number of new TB cases is around 8.8 million, and the number of deaths from TB is around 1.5 million. The number of infections by non-tuberculous mycobacteria (NTM) has been increasing in recent years. NTM symptoms can be similar to those caused by TB. It is critical to distinguish NTM infections from TB to determine the appropriate treatment because many NTM are resistant to ordinary antituberculous drugs. Definite diagnosis of TB is made by detecting M. tuberculosis from a clinical sample.

The Capilia TB-Neo point-of-care test is an immunochromatographic assay, which can give results for the identification of M. Tuberculosis from both liquid and solid mediums within 15 minutes. Because it can be stored at room temperature, it can also be used in areas with little to no access to laboratory equipment. Several publications also showed it has a very high specificity. Read more.


Taking Molecular Technology Personally


Presto CT/NG publication Rwanda

This year a new publication regarding CT/NG diagnosis with the Presto CT/NG 500 is published!

Abstract

BACKGROUND:

The Presto combined qualitative real-time assay for Chlamydia trachomatis and Neisseria gonorrhoeae (Presto CT/NG PCR assay) is appealing for developing countries, because it can be used with multiple DNA extraction methods and polymerase chain reaction (PCR) platforms.

OBJECTIVES:

The objective of the study was to implement and evaluate the Presto CT/NG PCR assay at the National Reference Laboratory (NRL) in Kigali, Rwanda, where no real-time PCR assays for the detection of C. trachomatis or N. gonorrhoeae were available.

METHODS:

The Presto CT/NG PCR assay was first evaluated at the Institute of Tropical Medicine (ITM) in Antwerp, Belgium. Next, NRL laboratory technicians were trained to use the assay on their ABI PRISM 7500 real-time PCR instrument and their competencies were assessed prior to trial initiation. During the trial, endocervical swabs were tested at the NRL, with bi-monthly external quality control testing monitored by the ITM. The final NRL results were evaluated against extended gold standard testing at the ITM, consisting of the Abbott m2000 RealTime System with confirmation of positive results by an in-house real-time PCR assay for C. trachomatis or N. gonorrhoeae.

RESULTS:

Of the 192 samples analysed using the Presto assay at the NRL, 16 samples tested positive for C. trachomatis and 17 tested positive for N. gonorrhoeae; four of these were infected with both. The sensitivity and specificity of the Presto assay were 93.3% (95% confidence interval [CI]: 68.1% – 99.8%) and 99.4% (95% CI: 96.8% – 100%) for C. trachomatis and 100% (95% CI: 76.8% – 100%) and 98.8% (95% CI: 95.8% – 99.9%) for N. gonorrhoeae.

CONCLUSION:

C. trachomatis and N. gonorrhoeae testing with the Presto assay was feasible in Kigali, Rwanda, and good performance was achieved.

R


Complimentary Products: Tauns Capilia TB-Neo

The Tauns Capilia TB-Neo point-of-care test gives results for identification of M. Tuberculosis within 15 minutes! More…


EDIT

Lactose intolerance PCR test (LCT 13910C>T)

The patient friendly solution to lactose intolerance testing

The Lactose intolerance is usually tested with a drink containing lactose. After two hours a blood sample will be taken to measure how much glucose is in the blood. If the blood glucose level does not rise, this means that the body is not digesting or absorbing lactose. This way of analyzing the lactose tolerance is very patient unfriendly.

A better solution would be to just take blood sample and run a molecular test. ViennaLab offers a LCT RealFast Assay. This PCR test tests on the LCT 13910C>T mutation.

Unique features of the RealFast Assays

The RealFast Assays from ViennaLab are a great product line. All RealFast assays can be combined in the same thermocylcer. This because the thermocycling program is identical for all RealFast Assays. this way the Lactose intolerance test can be combined with tests like HFE, FV and FII(PTH).

HLA-B27 PCR Test

HLA-B27 is a genetic risk factor for seronegative spondyloarthropathies, such as ankylosing spondylitis, reactive arthritis, juvenile rheumatoid arthritis and anterior uveitis.

The HLA-B27 PCR test from Viennalab is known for its high quality but especially for its exceptional speed. The very short PCR program ensures that the results are generated quickly and accurately. All Viennalab PCR tests can also be carried out with the same protocol. This means you do not have to save up your samples from various genetic analysis.  With the RealFast assays you can combine analysis like Haemachromatosis, Factor V leiden, PTH & HLA B27 in one PCR run.

Furthermore, the HLA-B27 PCR test can be combined with a verified fast and low priced DNA isolation kit, which means that the total costs can be kept interesting. More…

 

Comprehensive CE-IVD CYP2D6 assay

The cytochrome P450 2D6 (CYP2D6 assay) is an important liver enzyme involved in the metabolism of up to 25% of clinically used drugs (e.g. tamoxifen, opiates, anti-depressives or anti-psychotics). The CYP2D6 gene is highly polymorphic with over 100 allelic variants and numerous sub-variants described in the Human Cytochrom P450 Database (www.cypalleles.ki.se).

Request a FREE CYP2D6 kit!

While the most frequent allelic variations are caused by single nucleotide polymorphisms (SNPs) and small insertions or deletions, highly homologous regions in the CYP2D6 gene locus facilitate unequal cross-over leading to large deletions, duplications and gene conversions. Most of these alterations result in a change of the CYP2D6 enzyme activity. Guidelines recommend to determine the CYP2D6 genotype of patients prior to medication in order to be able to estimate the metabolic activity of the enzyme, and in consequence the clinical efficacy or even the applicability of defined drugs.

Please click below for the full article.

Comprehensive analysis of CYP2D6 variants and copy numbers using reverse-hybridization and real-time PCR based assays

Cystic Fibrosis Extended 38 mutations + 1 polymorphism

Cystic Fibrosis ExtendedThe extended CF is now available for the identification of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene mutations based on polymerase chain reaction (PCR) and reverse-hybridization. For human in vitro diagnostics.

The procedure includes three steps:

(1) DNA isolation

(2) PCR amplification using biotinylated primers

(3) hybridization of amplification products to test strips containing allele-specific oligonucleotide probes immobilized as an array of parallel lines.

Bound biotinylated sequences are detected using streptavidin-alkaline phosphatase and color substrates. The assay covers 38 mutations and 1 polymorphism in the CFTR gene: CFTRdel2,3 (21kb), I507del (-ATC), F508del (-CTT), 1677delTA, 1717-1G>A, G542X, G551D, R553X, 2043delG, 2143delT, 2183AA>G, 2184delA, 2184insA, 2789+5G>A, R1158X, R1162X, 3659delC, 3905insT, W1282X, N1303K [Teststrip A]; E60X, G85E, 394delTT, E92X, E92K, R117H, 621+1G>T, 1078delT, R334W, I336K, R347H, R347P, A455E, 3120+1G>A, 3272-26A>G, Y1092X, M1101K, 3849+10kbC>T, IVS8 5T/7T/9T [Teststrip B].

Manual: CF StripAssay 38+1 mutations

Liquid profiling enabled by Viennalab

ViennaLab is now releasing the first real-time PCR assay for monitoring lung cancer patients eligible to the alternative treatment with Osimertinib (Tagrisso) due to acquired resistance to EGFR-tyrosine kinase inhibitor (TKI) therapy: EGFR T790M RealFast Assay.
For proper sample preparation, ViennaLab is offering a column-based Plasma cfDNA Extraction Kit allowing isolation of high-purity circulating cell-free DNA from fresh or frozen human plasma.
The kits are available now!

Goffin at Scientific Spring Meeting Papendal (NVMM 2019)

Goffin Molecular Technologies invites you to visit us at stand 8 at the Scientific Spring Meeting KNVM & NVMM 2019 on March 26th and 27th. We will be promoting the Presto STI kits as well as Molzym products for 16S/18S/NGS purposes.

Please visit us for information and advice on the following subjects:

Check out on our booth no. 8,  the only one in the market SelectNA Plus automated pathogen 16S/18S DNA extraction instrument. 

The conference will be held at the Papendal Hotel and Conference Centre in Arnhem and will be hosted by the Dutch Society of Medical Microbiology (NVMM) and the Royal Dutch Society of Microbiology (KNVM).

For parking information and route description please click here