Lactose intolerance PCR test (LCT 13910C>T)

The patient friendly solution to lactose intolerance testing

The Lactose intolerance is usually tested with a drink containing lactose. After two hours a blood sample will be taken to measure how much glucose is in the blood. If the blood glucose level does not rise, this means that the body is not digesting or absorbing lactose. This way of analyzing the lactose tolerance is very patient unfriendly.

A better solution would be to just take blood sample and run a molecular test. ViennaLab offers a LCT RealFast Assay. This PCR test tests on the LCT 13910C>T mutation.

Unique features of the RealFast Assays

The RealFast Assays from ViennaLab are a great product line. All RealFast assays can be combined in the same thermocylcer. This because the thermocycling program is identical for all RealFast Assays. this way the Lactose intolerance test can be combined with tests like HFE, FV and FII(PTH).

HLA-B27 PCR Test

HLA-B27 is a genetic risk factor for seronegative spondyloarthropathies, such as ankylosing spondylitis, reactive arthritis, juvenile rheumatoid arthritis and anterior uveitis.

The HLA-B27 PCR test from Viennalab is known for its high quality but especially for its exceptional speed. The very short PCR program ensures that the results are generated quickly and accurately. All Viennalab PCR tests can also be carried out with the same protocol. This means you do not have to save up your samples from various genetic analysis.  With the RealFast assays you can combine analysis like Haemachromatosis, Factor V leiden, PTH & HLA B27 in one PCR run.

Furthermore, the HLA-B27 PCR test can be combined with a verified fast and low priced DNA isolation kit, which means that the total costs can be kept interesting. More…

 

Comprehensive CE-IVD CYP2D6 assay

The cytochrome P450 2D6 (CYP2D6 assay) is an important liver enzyme involved in the metabolism of up to 25% of clinically used drugs (e.g. tamoxifen, opiates, anti-depressives or anti-psychotics). The CYP2D6 gene is highly polymorphic with over 100 allelic variants and numerous sub-variants described in the Human Cytochrom P450 Database (www.cypalleles.ki.se).

Request a FREE CYP2D6 kit!

While the most frequent allelic variations are caused by single nucleotide polymorphisms (SNPs) and small insertions or deletions, highly homologous regions in the CYP2D6 gene locus facilitate unequal cross-over leading to large deletions, duplications and gene conversions. Most of these alterations result in a change of the CYP2D6 enzyme activity. Guidelines recommend to determine the CYP2D6 genotype of patients prior to medication in order to be able to estimate the metabolic activity of the enzyme, and in consequence the clinical efficacy or even the applicability of defined drugs.

Please click below for the full article.

Comprehensive analysis of CYP2D6 variants and copy numbers using reverse-hybridization and real-time PCR based assays

Cystic Fibrosis Extended 38 mutations + 1 polymorphism

Cystic Fibrosis ExtendedThe extended CF is now available for the identification of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene mutations based on polymerase chain reaction (PCR) and reverse-hybridization. For human in vitro diagnostics.

The procedure includes three steps:

(1) DNA isolation

(2) PCR amplification using biotinylated primers

(3) hybridization of amplification products to test strips containing allele-specific oligonucleotide probes immobilized as an array of parallel lines.

Bound biotinylated sequences are detected using streptavidin-alkaline phosphatase and color substrates. The assay covers 38 mutations and 1 polymorphism in the CFTR gene: CFTRdel2,3 (21kb), I507del (-ATC), F508del (-CTT), 1677delTA, 1717-1G>A, G542X, G551D, R553X, 2043delG, 2143delT, 2183AA>G, 2184delA, 2184insA, 2789+5G>A, R1158X, R1162X, 3659delC, 3905insT, W1282X, N1303K [Teststrip A]; E60X, G85E, 394delTT, E92X, E92K, R117H, 621+1G>T, 1078delT, R334W, I336K, R347H, R347P, A455E, 3120+1G>A, 3272-26A>G, Y1092X, M1101K, 3849+10kbC>T, IVS8 5T/7T/9T [Teststrip B].

Manual: CF StripAssay 38+1 mutations

Liquid profiling enabled by Viennalab

ViennaLab is now releasing the first real-time PCR assay for monitoring lung cancer patients eligible to the alternative treatment with Osimertinib (Tagrisso) due to acquired resistance to EGFR-tyrosine kinase inhibitor (TKI) therapy: EGFR T790M RealFast Assay.
For proper sample preparation, ViennaLab is offering a column-based Plasma cfDNA Extraction Kit allowing isolation of high-purity circulating cell-free DNA from fresh or frozen human plasma.
The kits are available now!

Goffin at Scientific Spring Meeting Papendal (NVMM 2019)

Goffin Molecular Technologies invites you to visit us at stand 8 at the Scientific Spring Meeting KNVM & NVMM 2019 on March 26th and 27th. We will be promoting the Presto STI kits as well as Molzym products for 16S/18S/NGS purposes.

Please visit us for information and advice on the following subjects:

Check out on our booth no. 8,  the only one in the market SelectNA Plus automated pathogen 16S/18S DNA extraction instrument. 

The conference will be held at the Papendal Hotel and Conference Centre in Arnhem and will be hosted by the Dutch Society of Medical Microbiology (NVMM) and the Royal Dutch Society of Microbiology (KNVM).

For parking information and route description please click here

New & Improved Molzym website

Molzym have launched their new website. The updated website includes navigation changes and much more.

The new website is also more compatible with mobile devices. All content is easily structured in dropdown menus for both desktop and mobile versionsPCR Related

Go there now!

Griepgolf? Vraag nu de 8 minuten Influenza sneltest aan!

Griep (Influenzavirus) is een aandoening van de luchtwegen veroorzaakt door Influenza A & B. Omdat deze virussen elk jaar verschillen bestaat er geen standaard vaccin voor. Griep kan erg lastig zijn voor de dagelijks functioneren. Het diagnosticeren van griep kan dan ook voordeel hebben voor het vroeg adequaat behandelen en reageren. Aangezien griep zeer besmettelijk is kunnen mensen in de directe omgeving hier ook rekening mee houden. Wanneer er een griepgolf actief is, kan het nuttig zijn om hiervan de diagnose te kunnen stellen. Veelal verwarren patiënten een verkoudheid met het griepvirus.

De Tauns Capilia Flu grieptest zorgt voor een snelle diagnose van zowel Influenza A en/of B. Door middel van een nasopharyngeale swab wordt een monster genomen, van dit monster is na 8 minuten resultaat.  Voor de test zijn geen aparte materialen of apparatuur nodig. Hierdoor biedt de Tauns grieptest de meest eenvoudige en snelle test voor een accurate diagnose.

Vraag hieronder een testkit aan!

 

Bestel nu een trial kit!

Point of care influenza A+B test

Unique point-of-care test for Influenza A+B. Easy and fast diagnosis with nasal of pharyngeal swab.

The Capilia™ Flu Neo test will give you a positive or negative result from nasal or pharyngeal swab in 8 minutes. The swab medium can also be used for other Capilia™ tests, such as RSV, hMPV and Adeno. No special instruments or equipment are required.

 

Because the Influenza virus is highly contagious, rapid diagnosis and prompt treatment can have a positive effect on public health. Also, the ability to distinguish between A or B can help physicians to prescribe an appropriate antiviral therapy.

Type A Sensitivity/Specificity: 94,3%/95,4% (From nasal swabs)

Type B Sensitivity/Specificity: 100%/98,7% (From nasal swabs)

 

The Capilia™ Flu Neo, as shown above, is based on an immunochromatography assay using a monoclonal antibody that recognizes influenza virus antigens. When a nasal or pharyngeal swab is taken, the swab medium is applied to the sample area. Result will follow in 8 minutes.

Point-of-care tests from Tauns laboratories

These unique products offer point-of-care diagnostics in minutes for laboratories and general practitioners, using only nasal and pharyngeal swabs as samples.

 

The Capilia™ product line by Tauns laboratories offers fast and sensitive POCT detection of pathogens like Influenza, RSV or hMPV.

Easy-to-use point of care strips, as shown below in the Flu Neo test.

 

  • Rapid test for detecting antigen: Permitting accurate results; simple and easy to use
  • Easy to read Black Line: Adopting the latest nanotechnology,”Platinum-Gold Colloid”
  • Fast test results: Reading time is 3-8 min.
  • Shared use of extraction buffer in Capilia series:  1 sample can be used for 4 types of tests(Flu,Adeno,RSV,hMPV)

 

Other Capilia™ products include Mycoplasma, TB and Strep A.