ViennaLab Newsletter

ViennaLab is now releasing the first multiplexseries of multiplex (mpx) RealFast™ Assays. The ViennaLab multiplex RealFast™ Assays allow to genotype two loci simultaneously and thus save costs and sample material.

The following multiplex RealFast™ Assays will become available as of 1st week of May 2018:

The CYP2C9 mpx RealFast™ Assay is designed for the simultaneous detection of the c.430C>T (CYP2C9*2) and c.1075A>C (CYP2C9*3) polymorphisms in the human CYP2C9 gene. Both variants of the CYP2C9 enzyme, *2 and *3, exhibit decreased function leading to poor metabolism (PM) phenotypes for various drugs. Patients with low enzyme activity are at risk of adverse drug reactions or therapeutic failure, particularly for CYP2C9 substrates with a narrow therapeutic window, such as S-warfarin or phenytoin. The kit is designed to identify patients carrying one or two variants of the CYP2C9 *2 or *3 variants.

The Alpha-1 antitrypsin (AAT) mpx RealFast™ Assay is designed for the simultaneous detection of the protease inhibitor (PI) variants *S and *Z of the SERPINA1 gene. These are the most frequent alleles associated with alpha-1 antitrypsin deficiency. The kit is intended for use as a diagnostic tool to identify alpha-1 antitrypsin deficient patients carrying a PI*S or PI*Z allele.

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3 Minute blood extraction kit

Arcis Biotechnology Arcis Blood Kit 48rxn is a ready to use kit comprising two pre-filled reagent tubes enabling extraction of nucleic acids from blood samples prior to a flexible range of downstream assays. 

The product is intended to be used by trained users proficient in molecular biological techniques. In 3 minutes, with no prior sample preparation, the kit allows you to go from blood to downstream nucleic acid investigations without the need for additional isolation or purification. The Arcis Blood Kit will release nucleic acids from fresh, dried or frozen whole blood samples. The kit is also suitable for specimens that have been stored in EDTA and heparin containing solutions. The nucleic acids released are ready for use in PCR or other molecular applications.

Please click  here for more information.


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Full DNA/RNA Isolation in 3 minutes

Arcis Biotechnology

The Arcis Pathogen Kit 48rxn is a ready to use kit comprising two pre-filled reagent tubes enabling the extraction of nucleic acids from a variety of biological samples. The product is intended to be used by trained users proficient in molecular biological techniques.

In 3 minutes, with no prior sample preparation, the Arcis Pathogen Kit allows you to go from bacterial samples to downstream nucleic acid investigations without the need for isolation or purification. The Arcis Pathogen Kit is an IVD sample prep system for the release of bacterial nucleic acids from bacteria grown on common microbiological growth substrates.  The material released is suitable for use in molecular diagnostic investigations such as PCR.  The product has been tested on the following sample types: bacterial cells on agar, liquid broth and standard laboratory buffers such as PBS.  Gram Positive and Gram negative bacteria have been used in validation studies including E.coli, S. aureus and K. pneumonia.

The Arcis DNA Pathogen Kit is intended for in vitro diagnostic use.  The Arcis Pathogen Kit can also be used in research (non IVD) to release DNA and RNA from pathogens in a variety od biological sample types including blood, swabs and saliva.

Please click  here for more information.


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Sample Prep Kit for any sample

Our new Sample Prep Kit 48rxn from Arcis is a ready to useArcis Biotechnology kit comprising two reagents tubes enabling flexible processing of a variety of sample types and sample volumes including whole blood, saliva, bacteria, plant or animal cells. The fast and convenient Arcis DNA Sample Prep kit allows you to go from cells to downstream nucleic acid investigations without the need for isolation or purification.

The product can be used on virtually any DNA or RNA containing sample. The nucleic acid released has been successfully applied to molecular biology techniques including PCR, RT-qPCR and sequencing. The ARCIS Sample Prep Kit accelerates pre-analytical processing in the following targets:

  • Human Samples: including whole blood (fresh/frozen, with and without heparin or EDTA), saliva, plasma, urine and buccal swabs
  • Microbiological samples: including bacteria (E.coli, S.aureus, P.aeruginosa, K. pneumoniae), viruses(HBV/HCV) and parasites (plasmodium)

Please click  here for more information.


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FV-PTH mpx PCR

FV and PTH are major FV-PTH PCRplayers in the coagulation cascade. The FV Leiden mutation causes a single amino acid exchange at position 506 (R506Q), which alters a cleavage site and thereby prevents efficient inactivation of FV. FV-PTH PCR

The FV-PTH mpx RealFast™ Assay is a fast and accurate multiplex real-time PCR test for the simultaneous detection of the two most important thrombophilic mutations. The point mutation 1691G>A in the human coagulation Factor V (F5) gene, referred to as FV Leiden, and the prothrombin (PTH) 20210G>A mutation in the Factor II (F2) gene are associated with hereditary thrombophilia.

The kit is intended to test patients suspected of having an increased risk for thrombotic disorders. The qualitative assay discriminates the three possible genotypes for each of the mutations in human genomic DNA: normal, heterozygous or homozygous mutant.

Please click  here for more information.

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Haemochromatosis

The easy way to test for Haemochromatosis 

Haemochromatosis

Hereditary haemochromatosis is a common genetic disorder of iron metabolism. It results in a progressive accumulation of iron in various organs, such as liver, heart and pancreas. Common complications include liver cirrhosis, diabetes, arthritis or cardiomyopathies.

Classical or type 1 haemochromatosis is caused by mutations in the HFE gene. They all code for proteins with an important role in regulating body iron stores, mutated forms can lead to iron accumulation in tissues and organs and can ultimately disrupt their normal functions.

The StripAssays® offer an easy way to identify the most common mutations in genes contributing to hereditary iron overload. Established innovations in diagnostics by ViennaLab thus aid in the early identification and effective therapy.

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PGX-5FU StripAssay

5-Fluorouracil (5-FU) is widely andPGX-5FU StripAssay successfully used for the treatment of solid tumors. In some patients, however, an enzyme variant results in the need of therapy adjustment. The PGX-5FU StripAssay identifies the most common mutation with therapeutic relevance for 5-FU treatment.

The ViennaLab PGX-5FU StripAssays®
• are based on reverse-hybridization of biotinylated PCR products
• combine probes for mutations and controls in a parallel array of allele-specific oligonucleotides
• work with immobilized oligos on a teststrip
• show mutations by enzymatic color reaction easily visible to the naked eye

Click here for more information

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Alpha-1 antitrypsin (AAT) cause of COPD

The Alpha-1 antitrypsin (AAT) mpx RealFast™ Assay for COPD diagnoses is designed for the simultaneous detection of the protease inhibitor (PI) variants *S and *Z of the SERPINA1 gene. These are the most frequent alleles associated with alpha-1 antitrypsin deficiency. The kit is intended for use as a diagnostic tool to identify alpha-1 antitrypsin deficient patients carrying a PI*S or PI*Z allele.

Alpha-1 antitrypsin deficiency is one of the most common hereditary disorders in persons of northern European heritage and is effecting approximately 3 million people and is a underrecognized cause of COPD! Deficient variants are characterized by low serum levels of AAT and, for some alleles including the Z allele, also decreased enzymatic function. Unhindered proteolysis in the lungs causes damage of alveolar tissue and development of chronic obstructive pulmonary disease (i.e. emphysema, persistent airflow obstruction, and/or chronic bronchitis). Accumulation of abnormal AAT in the liver leads to liver disease, especially cirrhosis and hepatocellular carcinoma. Individuals carrying the PI*ZZ genotype are at a high risk of developing emphysema and liver disease, whereas the PI*SZ genotype is associated with a somewhat lower risk to become symptomatic.

INFO

CYP2D6 StripAssay

CYP2D6, a member of the cytochrome CYP2D6 stripassayP450 superfamily, is one of the most important liver enzymes involved in the metabolism of xenobiotics in the body. Numerous drugs prescribed for the treatment of breast cancer (tamoxifen), cardiacdiseases(antiarrhythmics, beta-receptor blockers), psychiatric disorders (antipsychotics, antidepressants) or pain(opiates) are substrates for CYP2D6. CYP2D6 stripassay

The ViennaLab PGX-CYP2D6 StripAssay detects detects the most common variants with impaired enzyme activity. It detects the allelic variants CYP2D6*3 (2637delA), CYP2D6*4 (193 G>A) and CYP2D6*6 (1795delT).


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NVMM Scientific Spring Meeting 27 and 28 March 2018

This year we attended the NVMM Scientific Spring Meeting on 27 and 28 March 2018 with our team. We had a booth with our new and exclusive products including the automated system for host DNA depletion and pathogen DNA isolation SelectNA plus.

NVMM Scientific Spring Meeting

Team Goffin during NVMM meeting 2018

 

 

 

 

 

We would like to thank the organisation and everybody who visited and attended this meeting. We have had many interesting talks about all the new developments. It is always nice to see all the enthusiasm about our new and special products.

If you did not get the chance to get your questions answered, please feel free to contact us on info@goffinmt.com or check our page about this event.

http://www.goffinmoleculartechnologies.com/nvmm-27-28-march-2018/