Fast, simple and accurate detection of single nucleotide polymorphisms (SNPs) and copy number variations (CNVs).
The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes. The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore. During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye. This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.
- Fast and easy handling
- Less than 90 min from DNA to result
- Ready-to-use reagents
- Include controls for wild type and mutant genotypes
- Same protocol for all genotyping assays
- Compatible with many real-time PCR instruments
Haemochromatosis Rt-PCR Assay
|HFE C282Y||7-130||Detect common mutations in HFE gene|
|HFE H63D||7-140||Detect common mutations in the HFE gene|