HLA B57 PCR (B57/5701)

Fast, simple and accurate detection of single nucleotide polymorphisms (SNPs) and copy number variations (CNVs).

HLA B57 PCR ViennaLab

 

The ViennaLab HLA B57 PCR (B57/5701) RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes. The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore. During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye. This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.

Benefits:

  • Fast and easy handling
  • Less than 90 min from DNA to result
  • Ready-to-use reagents
  • Include controls for wild type and mutant genotypes
  • Same protocol for all genotyping assays
  • Compatible with many real-time PCR instruments

HLA Rt-PCR Assays

ProductCatalogApplication
HLA-B57017-610Detects the HLA-B57*:01 allele
HLA-B577-620Detects the HLA-B*27 allele

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