Mastermix 16S DNA Free

Avoidance of False Positive Results using Molzym’s Mastermix 16S

Mastermix 16/18S: Avoidance of FalsePositive Results A common drawback of PCR assays targeting broad range bacterial sequences is the contamination of amplification reagents by bacterial DNA. Consequences of Mastermix 16sthe DNA contamination will be false-positive results and a decrease in analytical sensitivity. Molzym‘s Mastermix 16/18S products are guaranteed free of contaminating DNA thus providing accurate results.

Ultra-Sensitive Detection

Mastermix 16S: Ultra-Sensitive Detection Mastermix 16S products assure strong amplification activity and accuracy, and thereby guarantee high sensitivity of bacteria detection. Mastermix 16S products can be used with all commercial PCR and Real-Time PCR cyclers including Roche, ABI, Bio-Rad, Stratagene and Corbett.

Mastermix 16S Products:

  • Mastermix 16S Basic

Mastermix 16S/18S Basic is suitable to amplify bacterial and fungal DNA. Mastermix 16S Basic is a 2.5x- concentrated solution, the final volume of the reaction mixture being 25 μl. The product contains all components necessary for a PCR run. For PCR runs, supplied MolTaq 16S, DNA-free water, custom primers and/or probes and the template have to be added to obtain a complete reaction mixture.

Protocol: Mastermix 16S Basic – DNA Free. For the PCR amplification of bacterial and fungal DNA using custom primers.

 

 

Content Mastermix 16S Basic

Mastermix 16S Basic100 rxn.250 rxn.1.000 rxn.
2.5 x mastermix (3mM Mg*2+ final concentration)2 x 0.5 ml5 x 0.5 ml20 x 0.5 ml
MolTaq 16S DNA Polymerase (non-Hot Start)0.08 ml0.2 ml4 x 0.2 ml
DNA-free PCR-grade water1.7 ml3 x 1.7 ml10 x 1.7 ml
  • Mastermix 16S Primer

Mastermix 16S Primer contains validated primers binding to conserved regions of the 16S rRNA gene. The mastermix is suitable to amplify any eubacterial DNA. The amplified region (approx. 450 bp) contains variable sequences for the identification of bacteria by taxon specific probing or sequence analysis. Mastermix 16S Primer is a 2.5x-concentrated solution, the final volume of the reaction mixture being 25 μl. The product contains all components necessary for a PCR run. Only supplied MolTaq 16S, DNA-free water and the template have to be added to obtain a complete reaction mixture for PCR.

Protocol: Mastermix 16S Primer – Ultra Sensivity Detection of Microbial DNA. For the PCR amplification of bacterial DNA targets using universal 16S rDNA primers.

 

Content Mastermix 16S Primer DNA Free

Mastermix 16S Primer100 rxn.250 rxn.1.000 rxn.
2.5 x mastermix (3 mM MgCl*2 final concentration), incl. primers2 x 0.5 ml5 x 0.5 ml20 x 0.5 ml
MolTaq 16S DNA Polymerase (non-Hot Start)0.08 ml0.2 ml4 x 0.2 ml
DNA-free PCR-grade water1.7 ml3 x 1.7 ml12 x 1.7 ml
  • Mastermix 16S Dye

Mastermix 16S/18S Dye is designed to run PCR reactions with custom primers aimed to detect bacterial or fungal targets. The mastermix is suitable to amplify microbial DNA with added unspecific or specific primers and thereby detect the presence of bacteria or fungi in a sample. Detection of amplified DNA is done by gel electrophoresis, using components supplied with this kit (SYBR® Green 1 DNA staining solution for visualisation, DNA marker for size estimation of the amplicon, gel loading solution).

Mastermix 16S Dye may also be used for detection by Real-Time PCR with intercalating fluorescent dyes or probes or by array technologies. The amplicon may be used for the identification of microorganisms by taxon specific probing or sequence analysis. Mastermix 16S Dye is a 2.5x-concentrated solution, the maximum final volume of the reaction mixture being 25 μl. The product contains all components necessary for a PCR run. For PCR runs, supplied MolTaq 16S, DNA-free water, custom primers and/or probes and the template have to be added to obtain a complete reaction mixture.

Protocol: Mastermix 16S Dye – DNA Free. For the PCR detection and identification of bacterial or fungal DNA targets with custom primers.

 

Content Mastermix 16S Dye

Mastermix 16S Dye100 rxn.250 rxn.1.000 rxn.
2.5 x mastermix (3 mM Mg*2+ final concentration)2 x 0.5 ml5 x 0.5 ml20 x 0.5 ml
MolTaq 16S DNA Polymerase (non-Hot Start)0.08 ml0.2 ml4 x 0.2 ml
DNA-free PCR-grade water1.7 ml3 x 1.7 ml10 x 1.7 ml
DNA staining solution, 10x concentrated0.25 ml0.625 ml4 x 0.625 ml
Gel loading solution, 6x concentrated0.2 ml0.5 ml2 ml
DNA size marker (1 kb ladder) pre-stained0.05 ml0.125 ml0.5 ml
  • Mastermix 16S Complete

Mastermix 16S/18S Complete contains validated primers binding to conserved regions of the 16S rRNA gene. The mastermix is suitable to amplify any eubacterial DNA and thereby detect the presence of bacteria in a sample. Detection of amplified DNA is done by gel electrophoresis, using components supplied with this kit (SYBR® Green 1 DNA staining solution for visualisation, DNA marker for size estimation of the amplicon, gel loading solution).

Mastermix 16S Complete may also be used for detection by Real-Time PCR with intercalating fluorescent dyes or probes or by array technologies. The amplified region (approx. 450 bp) contains variable sequences for the identification of bacteria by taxon specific probing or sequence analysis. Mastermix 16S Complete is a 2.5x-concentrated solution, the maximum final volume of the reaction mixture being 25 μl. The product contains all components necessary for a PCR run. Only supplied MolTaq 16S, DNA-free water and the template have to be added to obtain a complete reaction mixture for PCR.

Protocol: Mastermix 16S Complete – DNA Free. For the PCR detection and identification of bacteria using universal 16S rDNA primers.

 

Content Mastermix 16S Complete

Mastermix 16S Complete100 rxn.250 rxn.1.000 rxn.
2.5 x mastermix (3 mM MgCl*2 final concentration), incl. primers2 x 0.5 ml5 x 0.5 ml20 x 0.5 ml
MolTaq 16S DNA Polymerase (non-Hot Start)0.08 ml0.2 ml4 x 0.2 ml
DNA-free PCR-grade water1.7 ml3 x 1.7 ml12 x 1.7 ml
DNA staining conclusion, 10x concentrated0.25 ml0.625 ml4 x 0.625 ml
Gel loading solution, 6x concentrated0.2 ml0.5 ml4x 0.5 ml
DNA size marker (1 kb), pre-stained0.05 ml0.125 ml4 x 0.125 ml

Ordering information Mastermix 16S DNA free

ProductCatalogCapacity
Mastermix 16S CompleteS-020-0100100 reactions
Mastermix 16S CompleteS-020-0250250 reactions
Mastermix 16S CompleteS-020-10001000 reactions
Mastermix 16S PrimerS-021-0100100 reactions
Mastermix 16S PrimerS-021-0250250 reactions
Mastermix 16S PrimerS-021-10001000 reactions
Mastermix 16S DyeS-030-0100100 reactions
Mastermix 16S DyeS-030-0250250 reactions
Mastermix 16S DyeS-030-10001000 reactions
Mastermix 16S BasicS-040-0100100 reactions
Mastermix 16S BasicS-040-0250250 reactions
Mastermix 16S BasicS-040-10001000 reactions

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