In case of the PCR method amplified by using general Taq DNA Polymerase, ideal length of an amplified fragment is less than 1 Kb and not greater than 3 Kb in theory. Over 10 Kb fragments can be amplified by a standard PCR technique, but it is hard to get high amplification efficiency and reliable results with longer fragments.
PCR technology has been developed to get reliable results and high amplification efficiency for these long PCR fragments. Discovery of pfu DNA Polymerase has had a biggest effect on widely known PCR methods for amplifying 20 Kb ~ 40 Kb. pfu DNA Polymerase is isolated from P. furiosis and has proof-reading function. To carry out long PCR experiment, 2x PCR Master mix Solution(i-StarMAXTM GH) is can be a suitable Kit. The 2x PCR Master mix Solution(i-StarMAXTM GH) is used i-StarMAXTM GH DNA Polymerase, which is containing Taq DNA Polymerase and thermostable DNA Polymerase with proofreading activity.
The advantage of i-StarMAXTM GH DNA Polymerase is the outstanding PCR accuracy compared to general Taq DNA Polymerase. The i-StarMAXTM GH DNA Polymerase has proof-reading function which automatically stops to synthesize nucleotide sequence when incorrect PCR fragment interrupts template. But it is only applied to long PCR and there is a drawback that performance time of PCR is longer.
So, 2x PCR Master mix Solution(i-StarMAXTM GH) to make up for this weakness, and then 2X PCR Master Mix Solution (i-StarMAX™ GH) is designed to get the best result with simple use that all components are premixed in a tube with 2X solution mix type. After adding template DNA, primer set and D.W. only, performing PCR is possible and loading gel is done with no any other treatment because of gel loading buffer.
2x PCR Master mix Solution (i-StarMAX™ GH) using i-StarMAX™ GH DNA Polymerase has convenience as well as high specificity and guarantees the reliable result and the reproducibility for various samples.
PCR 2X Master Mix
|2X Master/MultiMAX PCR Kit||25501||.5 mℓ x 2 vials||General PCR/Hot-Start/Multiplex PCR|
|2X Master/MaHa7™ PCR (HS)||25451||.5 mℓ x 2 vials||General PCR/Hot-Start/Fast PCR|
|2X PCR Master Mix Solution (i-Taq)||25027||.5 mℓ x 2 vials||General PCR|
|2X PCR Master Mix Solution (i-Taq)||25028||1 mℓ x 5 vials||General PCR|
|2X PCR Master Mix Solution (i-StarTaq)||25166||.5 mℓ x 2 vials||General PCR/Hot-Start|
|2X PCR Master Mix Solution (i-StarTaq GH)||26032||.5 mℓ x 2 vials||General PCR/Hot-Start|
|2X PCR Master Mix Solution (i-pfu)||25186||.5 mℓ x 2 vials||General PCR/High Fidelity|
|2X PCR Master Mix Solution (i-MAX ll)||25266||.5 mℓ x 2 vials||General PCR/Long/High Fidelity|
|2X PCR Master Mix Solution (i-StarMAX ll)||25174||.5 mℓ x 2 vials||General PCR/Hot-Start/Long/High Fidelity|
|2X PCR Master Mix Solution (i-StarMAX GH)||26041||.5 mℓ x 2 vials||General PCR/Hot-Start/Long/High Fidelity|