Molecular analysis of pathogenic bacteria and fungi in liquid samples from clinical materials and animal systems (e.g., blood and other body liquids) can be severely disturbed by a high background of host DNA. MolYsis™ Basic5 removes this background of host DNA and thereby increases the reliability of the molecular analysis of pathogens in clinical and other samples. The kit contains all ingredients for the selective lysis of host cells and the degradation of released nucleic acids (DNA and RNA) in samples. Patented MolYsis™ Basic5 is a sample pre-treatment tool for the removal of host as well as dead cell DNA. The kit can be used as a module in conjunction with any other nucleic acid extraction kit designed for handling in the mini bind-wash-elute format (e.g., mini spin columns, automated systems).
Molzym also supplies kits, manual MolYsis™ Complete5 (1 and 5ml; D-321-050) and MolYsis™ Complete10 (5 to 10ml; D-325-050) and automated SelectNA Blood Pathogen Kit (1ml; D-340-048), for the complete process of microbial DNA isolation from clinical and other samples, including sample pre-treatment, enrichment and lysis of bacterial and fungal cells, DNA extraction and DNA purification. Whereas other systems result in a mixture of host and microbial DNA, sample pretreatment tool, MolYsis™ Basic5, enables the preparation of microbial DNA from samples. Only two steps are needed to obtain a sample that is depleted of host and dead cell DNA
Fig. 1. The principle of testing for bacterial and fungal pathogens in liquid samples by MolYsis™ Basic 5 and other Kits for the purification of pathogen DNA.
- Sepsis diagnosis of adult patients
- Infection diagnosis of other body fluids, including cerebrospinal fluids and joint aspirates
- MolDNase (DNA degradation)
- Buglysis (degradation of Gram-positive and Gram-negative cell walls)
APPLICATION NOTE: MOLYSIS – MICROBIAL DNA ISOLATION FROM DIVERSE CLINICAL SPECIMENS FOR SENSITIVE MOLECULAR DIAGNOSIS.
Ordering Information MolYsis (click on the product for manual)
|Molysis Basic||D-300-050||50 reactions|
|Molysis Basic||D-300-100||100 reactions|
|Molysis Basic 5||D-301-050||50 reactions|
|Molysis Basic 5||D-301-100||100 reactions|
|Molysis Basic 10||D-305-050||50 reactions|
|Molysis Basic 10||D-305-100||100 reactions|
|Molysis Complete 5||D-321-050||50 reactions|
|Molysis Complete 5||D-321-100||100 reactions|
|Molysis Complete 10||D-325-050||50 reactions|
|Molysis Complete 10||D-325-100||100 reactions|
|Molysis Plus||D-310-050||50 reactions|
|Molysis Plus||D-310-100||100 reactions|