Sample pre-treatment and bacterial/fungal DNA isolation kit for background-free PCR analysis of whole blood and other liquid samples for large size sample volumes (5 to 10ml).
With MolYsis Complete 10 Molzym has developed a tool for the enrichment of bacterial and fungal cells and isolation of pure microbial DNA from blood and other liquids. MolYsis Complete 10 is the complete solution for the removal of PCR inhibitors and host and dead cell DNA from samples, allowing the reliable and sensitive detection of bacterial and fungal pathogens through PCR or Real-Time PCR. MolYsi Complete 10 allows for the microbial DNA isolation from 5 to 10ml liquid samples
MolYsis Complete 10 is Molzym’s proprietary, patented technology enabling the enrichment and purification of microbial DNA from liquid clinical samples and animal model material for molecular analysis. The procedure includes protocols for i) human/animal DNA removal, ii) universal lysis of Gram-negative and Gram-positive bacteria, and fungi and iii) isolation of the microbial DNA.
Only three steps are needed to obtain microbial DNA preparations that are depleted of host DNA (Fig. 1):
I) The addition of a chaotropic buffer to a liquid sample lyses the host cells, whereas microbial cells are unaffected. The DNA released from host cells as well as dead cells is degraded by Molzym’s proprietary, chaotrope-resistant MolDNase B.
II) Microbial cells are sedimented, treated with BugLysis reagents to degrade cell walls of Gram-negative bacteria, Gram-positive bacteria and fungi and then digested by Proteinase K treatment.
III) The microbial DNA is extracted and then isolated by a quick bind-wash-elute procedure, using Molzym’s CCT technology with quantitative DNA binding to the filter matrix and high recovery of microbial DNA from the column.
Fig. 1: The principle of testing for bacterial and fungal pathogens in samples by MolYsis™ Complete 10.
Applications Molysis Complete 10:
– Sepsis diagnosis of adult patients (10 ml protocol)
– Sepsis diagnosis of adult patients (5 ml protocol)
– Sepsis diagnosis of child patients (1 ml protocol)
– Infection diagnosis of other body fluids, including cerebrospinal fluids and joint aspirates
- MolDNase (DNA degradation)
- BugLysis (degradation of Gram-positive and Gram-negative cell walls)
- Mini spin columns
- 10.0 ml blood (extra protocol for 1.0 ml and 5.0 ml included)
APPLICATION NOTE: MOLYSIS – MICROBIAL DNA ISOLATION FROM DIVERSE CLINICAL SPECIMENS FOR SENSITIVE MOLECULAR DIAGNOSIS.
Ordering Information MolYsis (click on the product for manual)
|Molysis Basic||D-300-050||50 reactions|
|Molysis Basic||D-300-100||100 reactions|
|Molysis Basic 5||D-301-050||50 reactions|
|Molysis Basic 5||D-301-100||100 reactions|
|Molysis Basic 10||D-305-050||50 reactions|
|Molysis Basic 10||D-305-100||100 reactions|
|Molysis Complete 5||D-321-050||50 reactions|
|Molysis Complete 5||D-321-100||100 reactions|
|Molysis Complete 10||D-325-050||50 reactions|
|Molysis Complete 10||D-325-100||100 reactions|
|Molysis Plus||D-310-050||50 reactions|
|Molysis Plus||D-310-100||100 reactions|