Molysis Complete 10 the complete solution

Molzym Molysis Complete 10

Sample pre-treatment and bacterial/fungal DNA isolation kit for background-free PCR analysis of whole blood and other liquid samples for large size sample volumes (5 to 10ml).

With MolYsis Complete 10 Molzym has developed a tool for the enrichment of bacterial and fungal cells and isolation of pure microbial DNA from blood and other liquids. MolYsis Complete 10 is the complete solution for the removal of PCR inhibitors and host and dead cell DNA from samples, allowing the reliable and sensitive detection of bacterial and fungal pathogens through PCR or Real-Time PCR. MolYsi  Complete 10 allows for the microbial DNA isolation from 5 to 10ml liquid samples

MolYsis Complete 10 is Molzym’s proprietary, patented technology enabling the enrichment and purification of microbial DNA from liquid clinical samples and animal model material for molecular analysis. The procedure includes protocols for i) human/animal DNA removal, ii) universal lysis of Gram-negative and Gram-positive bacteria, and fungi and iii) isolation of the microbial DNA.

Only three steps are needed to obtain microbial DNA preparations that are depleted of host DNA (Fig. 1):

I) The addition of a chaotropic buffer to a liquid sample lyses the host cells, whereas microbial cells are unaffected. The DNA released from host cells as well as dead cells is degraded by Molzym’s proprietary, chaotrope-resistant MolDNase B.

II) Microbial cells are sedimented, treated with BugLysis reagents to degrade cell walls of Gram-negative bacteria, Gram-positive bacteria and fungi and then digested by Proteinase K treatment.

III) The microbial DNA is extracted and then isolated by a quick bind-wash-elute procedure, using Molzym’s CCT technology with quantitative DNA binding to the filter matrix and high recovery of microbial DNA from the column.

Fig. 1: The principle of testing for bacterial and fungal pathogens in samples by MolYsis™ Complete 10.

Applications Molysis Complete 10:

– Sepsis diagnosis of adult patients (10 ml protocol)
– Sepsis diagnosis of adult patients (5 ml protocol)
– Sepsis diagnosis of child patients (1 ml protocol)
– Infection diagnosis of other body fluids, including cerebrospinal fluids and joint aspirates

Contents:

  • Buffers
  • MolDNase (DNA degradation)
  • BugLysis (degradation of Gram-positive and Gram-negative cell walls)
  • Mini spin columns

Sample:

  • 10.0 ml blood (extra protocol for 1.0 ml and 5.0 ml included)

molysisapp pic2APPLICATION NOTE: MOLYSIS – MICROBIAL DNA ISOLATION FROM DIVERSE CLINICAL SPECIMENS FOR SENSITIVE MOLECULAR DIAGNOSIS.

 

 

Ordering Information MolYsis (click on the product for manual)

ProductCatalogContent
Molysis BasicD-300-05050 reactions
Molysis Basic D-300-100100 reactions
Molysis Basic 5D-301-05050 reactions
Molysis Basic 5D-301-100100 reactions
Molysis Basic 10D-305-05050 reactions
Molysis Basic 10D-305-100100 reactions
Molysis Complete 5D-321-05050 reactions
Molysis Complete 5D-321-100100 reactions
Molysis Complete 10D-325-05050 reactions
Molysis Complete 10D-325-100100 reactions
Molysis PlusD-310-05050 reactions
Molysis PlusD-310-100100 reactions

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