Molysis Plus sample pre-treatment kit

Molysis PlusMolzym Molysis Plus kit:

Especially designed for the isolation of bacterial DNA from small (0.2 ml) blood cultures. The kit is based on a proprietary technology for the efficient removal of strong PCR inhibitors from the medium and the isolation of extremely pure bacterial DNA for PCR and qPCR analysis.

MolYsis™ Plus is Molzym’s proprietary, patented technology enabling the purification of microbial DNA for PCR analysis from blood culture. The procedure includes protocols for:

i) Human blood DNA removal.

ii) Universal lysis of Gram-negative and Gram-positive bacteria, and fungi.

iii) Isolation of PCR inhibitor-free bacterial DNA.

Only three steps are needed to obtain bacterial DNA preparations that are depleted of host DNA. The addition of a chaotropic buffer to a liquid sample lyses the host cells, whereas bacterial cells are unaffected. The DNA released from host cells as well as dead cells is degraded by Molzym’s proprietary, chaotrope-resistant MolDNase A. Than the bacterial and fungal cells are sedimented, treated with BugLysis reagent to degrade cell walls of Gram-negative and Gram-positive bacteria and fungi and then digested by Proteinase K treatment. At last the bacterial DNA is extracted and then isolated by a quick bind-wash-elute procedure, using Molzym’s CCT technology with DNA binding to the filter matrix and high recovery of pure microbial DNA from the column.

Molysis Plus

Fig. 1: The principle of testing for bacterial and fungal pathogens in blood culture samples by MolYsis™ Plus

Applications:

  • Sepsis diagnosis of adult patients
  • Infection diagnosis of other body fluids, including cerebrospinal fluids and joint aspirates

Contents:

  • Buffers
  • MolDNase (DNA degradation)
  • Buglysis (degradation of Gram-positive and Gram-negative cell walls)

molysisapp pic2APPLICATION NOTE: MOLYSIS – MICROBIAL DNA ISOLATION FROM DIVERSE CLINICAL SPECIMENS FOR SENSITIVE MOLECULAR DIAGNOSIS.

 

 

Ordering Information MolYsis (click on the product for manual)

ProductCatalogContent
Molysis BasicD-300-05050 reactions
Molysis Basic D-300-100100 reactions
Molysis Basic 5D-301-05050 reactions
Molysis Basic 5D-301-100100 reactions
Molysis Basic 10D-305-05050 reactions
Molysis Basic 10D-305-100100 reactions
Molysis Complete 5D-321-05050 reactions
Molysis Complete 5D-321-100100 reactions
Molysis Complete 10D-325-05050 reactions
Molysis Complete 10D-325-100100 reactions
Molysis PlusD-310-05050 reactions
Molysis PlusD-310-100100 reactions

PRICE INQUIRY

Total Overview All Molzym Products