Especially designed for the isolation of bacterial DNA from small (0.2 ml) blood cultures. The kit is based on a proprietary technology for the efficient removal of strong PCR inhibitors from the medium and the isolation of extremely pure bacterial DNA for PCR and qPCR analysis.
MolYsis™ Plus is Molzym’s proprietary, patented technology enabling the purification of microbial DNA for PCR analysis from blood culture. The procedure includes protocols for:
i) Human blood DNA removal.
ii) Universal lysis of Gram-negative and Gram-positive bacteria, and fungi.
iii) Isolation of PCR inhibitor-free bacterial DNA.
Only three steps are needed to obtain bacterial DNA preparations that are depleted of host DNA. The addition of a chaotropic buffer to a liquid sample lyses the host cells, whereas bacterial cells are unaffected. The DNA released from host cells as well as dead cells is degraded by Molzym’s proprietary, chaotrope-resistant MolDNase A. Than the bacterial and fungal cells are sedimented, treated with BugLysis reagent to degrade cell walls of Gram-negative and Gram-positive bacteria and fungi and then digested by Proteinase K treatment. At last the bacterial DNA is extracted and then isolated by a quick bind-wash-elute procedure, using Molzym’s CCT technology with DNA binding to the filter matrix and high recovery of pure microbial DNA from the column.
Fig. 1: The principle of testing for bacterial and fungal pathogens in blood culture samples by MolYsis™ Plus
- Sepsis diagnosis of adult patients
- Infection diagnosis of other body fluids, including cerebrospinal fluids and joint aspirates
- MolDNase (DNA degradation)
- Buglysis (degradation of Gram-positive and Gram-negative cell walls)
APPLICATION NOTE: MOLYSIS – MICROBIAL DNA ISOLATION FROM DIVERSE CLINICAL SPECIMENS FOR SENSITIVE MOLECULAR DIAGNOSIS.
Ordering Information MolYsis (click on the product for manual)
|Molysis Basic||D-300-050||50 reactions|
|Molysis Basic||D-300-100||100 reactions|
|Molysis Basic 5||D-301-050||50 reactions|
|Molysis Basic 5||D-301-100||100 reactions|
|Molysis Basic 10||D-305-050||50 reactions|
|Molysis Basic 10||D-305-100||100 reactions|
|Molysis Complete 5||D-321-050||50 reactions|
|Molysis Complete 5||D-321-100||100 reactions|
|Molysis Complete 10||D-325-050||50 reactions|
|Molysis Complete 10||D-325-100||100 reactions|
|Molysis Plus||D-310-050||50 reactions|
|Molysis Plus||D-310-100||100 reactions|