Without DNA free reagents and plastics there is a strong risk of false positive signals as a cause of amplification from contaminating DNA in PCR reagents, including the polymerising enzyme, primers and dNTPs.
Molzym’s purification technology removes traces of DNA from the PCR reagents and supplies a highly active enzyme in the products. This results in enhanced sensitivity and reliability of bacteria and fungi detection in the assays.
Solutions are offered to meet your requirements ranging from DNA-free Taq polymerase, MolTaq 16S/18S to mastermixes in its various designs, Mastermix 16S and Mastermix 18S kits. Assays are available, Mastermix 16S Primer and Mastermix 16S Complete, for the detection of bacteria, and Mastermix 18S Primer and Mastermix 18S Complete, for the detection of fungi by the amplification of a highly variable regions of the 16S and 18S rRNA gene, respectively.
If you want to use your own primers, we offer Mastermix 16S/18S Basic and Mastermix 16S/18S Dye kits which contain all components for the PCR reaction except primers.
Sequencing of amplicons combined with online homology BLAST search is an option for the identification of bacteria and fungi detected by Molzym assays, Mastermix16S/18S Complete or Mastermix 16S/18S Primer. Molzym offers sequencing primers that allow the sequence analysis and identification of bacteria and fungi. The primers bind to conserved regions within the amplified DNA enabling sequencing of highly discriminative sequences for strain identification. Sequencing primer SeqGP16 covers a variety of Gram-positive organisms, including staphylococci, streptococci and enterococci. Sequencing primer SeqGN16 on the other hand allows the identification of many pathogenic Gram-negative bacteria, including enterobacteria and pseudomonads. Ask us for the up-to-date list of pathogens!
Sequences generated by using the sequencing primers can be easily analysed by referring to our free sequence alignment software, SepsiTest™ BLAST.
Application Note: MolTaq 16S/18S – DNA Free Taq Polymerase for 40-Cycles PCR Analysis of Low Load Microbial DNA Targets.
DNA-free Water for all PCR purposes
|DNA free water, PCR grade||P-020-0003||10 x 1.7ml|
Mastermix 16S DNA free
|Mastermix 16S Complete||S-020-0100||100 reactions|
|Mastermix 16S Complete||S-020-0250||250 reactions|
|Mastermix 16S Complete||S-020-1000||1000 reactions|
|Mastermix 16S Primer||S-021-0100||100 reactions|
|Mastermix 16S Primer||S-021-0250||250 reactions|
|Mastermix 16S Primer||S-021-1000||1000 reactions|
|Mastermix 16S Dye||S-030-0100||100 reactions|
|Mastermix 16S Dye||S-030-0250||250 reactions|
|Mastermix 16S Dye||S-030-1000||1000 reactions|
|Mastermix 16S Basic||S-040-0100||100 reactions|
|Mastermix 16S Basic||S-040-0250||250 reactions|
|Mastermix 16S Basic||S-040-1000||1000 reactions|