Ureaplasma PCR

Ureaplasma PCR AB analiticaUreaplasma PCR (RQ-STI-UUUP) kit for detection of Ureaplasma urealyticum and Ureaplasma parvum

Sexually transmitted infections (STI) comprise a large group of pathogen infections that account for about 340 million newly infected individuals every year. Although most infections occur in the developing countries, STI are also very common in the developed countries of Western Europe and the USA.STI-UUUP

Ureaplasma urealyticum (U. urealyticum o UU) and Ureaplasma parvum (U. parvum o UP) are bacteria that are often found in the urinary tract. Approx. 40 % to 80 % of women and 50 % of men carry these bacteria. Although many infections are asymptomatic, both species are responsible for a variety of pathological conditions. Ureaplasma urealyticum is mainly associated with nongonococcal urethritis and cystitis.

Ureaplasma parvum is more commonly found in pregnant women, where it may cause chorioamnionitis, which can lead to preterm birth, as well as respiratory and/or neurological complications in the newborn. These two species cannot be distinguished using the classical method of cell culture. Only with molecular biology methods, like the Real-Time PCR, the clinician has the means at hand to unequivocally identify those two pathogens.

Compatible platforms: Validated on

  • Applied Biosystems 7500 Fast / Fast Dx and StepOnePlus / StepOne Real-Time PCR System
  • Dx Real-Time System and CFX96 Real-Time PCR Detection System (Bio-Rad)
  • Analytical specificity: No non-specific pairing of primers and probes, no cross-reaction Analytical sensitivity (detection limit): 0.62 bacterial genome copies / µL DNA extract for UU and 0.44 bacterial genome copies / µL DNA extract for UP.

Specifications:

  • Diagnostic specificity: 100 %
  • Diagnostic sensitivity: 100 %
  • Accuracy: 100 %

The Ureaplasma PCR kit includes:

  • Ready-to-use reagents for Real-Time PCR
  • Amplification control (amplification of beta-globin gene – BG)
  • Positive control (DNA fragments of the Ureaplasma urealyticum and Ureaplasma parvum genome and the BG gene)
  • dUTP/UNG system for preventing carry-over contamination

RQ-STI-UU/UP

ProductCat.Capacity
REALQUALITY RQ-STI UU/UP
REALQUALITY RQ-STI UU/UP
RQ-73-48
RQ-73-96
48 test
96 test

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