Viennalab StripAssays® & RealFastTM Assays
ViennaLab is an Austrian company with a successful history of more than 20 years in developing, manufacturing and worldwide distributing genetic tests. StripAssays® based on reverse hybridization have up to 48 immobilised probes for wild-type and mutated alleles, and are accurate and reliable. RealFastTM Assays are based on real-time PCR and detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) fast and simply.
Viennalab offerts tests for Cardiovascular Diseases, Thalassemia, Familial Mediterranean Fever, Haemochromatosis, Gaucher Disease, Alzheimer Disease, Sugar (Lactose, Fructuose) Intolerance, Congenital Adrenal Hyperplasia, Cystic Fibrosis, KRAS, BRAF, among others. Products of the pharmacogenetics and oncology portfolio help to achieve safer and more individualised anticoagulant and cancer therapies.
Viennalab Quality Management System:
All products are designed and manufactured according to the quality standards of ISO 9001, ISO 13485, and are CE/IVD marked. The tests are also used in many international research studies and quality assessment schemes, which allow scientific evidence of the quality employed.
Technology behind StripAssays®:
The ViennaLab StripAssays® based on reverse hybridization carry up to 48 immobilised probes for wild-type and mutated alleles.
Step 1: Isolation of genomic DNA from bodily fluids, tissue or cells.
Step 2: Multiplex PCR amplification of specific DNA sequences and simultaneous biotin-labelling.
Step 3: Precise selective hybridization of specific sequences onto StripAssay®
Step 4: Easy and clear identification of hybridized sequences of interest only. Biotinylated sequences are detected by streptavidin-alkaline phosphatase.
Step 5: Automated result interpretation possible.
Technology behind RealFast™ Assays:
The ViennaLab RealFast™ Assays are based on Real-Time PCR and detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) fast and simply.
The Viennalab Principle:
– The ViennaLab RealFast™ Assays are based on Real-Time PCR and hydrolysis probes, also commonly called TaqMan® probes.
– The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5′-end and a quencher dye at the 3′-end.
– While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5′-fluorophore.
– During the combined annealing/extension phase of PCR, the probe is cleaved by the 5′ to 3′ exonuclease activity of Taq DNA Polymerase, thereby separating the fluorophore from the quencher dye.
– This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR products.