Introducing Imegen-Cambridge II: Your Solution for Detecting the SERPINC1 Gene Mutation Associated with Cambridge II Syndrome

Product Description: Imegen-Cambridge II is a cutting-edge molecular biology kit designed for the precise detection of the c.1246G>T (p.Ala416Ser) allele within the SERPINC1 gene, which is strongly linked to Cambridge II Syndrome—a hereditary thrombophilia characterized by a predisposition to thrombosis. This innovative kit employs real-time PCR technology to deliver accurate results and comes with 48 reaction tubes, making it a powerful tool for researchers in the field of genetics.

Key Features:

• Format: 48 reactions
• Technology: Real-time PCR
• Reference: IMG-199
• Compatible Equipment: 7500 FAST Real-Time PCR System (ThermoFisher), StepOnePlus Real-Time PCR System (ThermoFisher), StepOne Real-Time PCR System (Thermo Fisher)

Components Included:

1. Cambridge II Master Mix: This component contains specific oligonucleotides, labeled probes, and water reagents optimized for PCR reactions targeting the SERPINC1 gene mutation.
2. General Master Mix: A conventional PCR enzyme mix containing 10-30% glycerol, <0.1 sodium azide, and buffers.

Product Information: Cambridge II Syndrome is a hereditary thrombophilia that results in a deficiency of antithrombin enzyme (AT), a crucial regulator of the blood coagulation process. AT, synthesized in the liver and encoded by the SERPINC1 gene, belongs to the serpin superfamily and is known for its inhibitory activity on serine protease enzymes. AT plays a pivotal role in regulating clot formation by inhibiting the activity of thrombin and other coagulation-promoting proteins.

The Cambridge II variant, A368S, affects AT’s function, altering its ability to inhibit coagulation factors and thrombin compared to the non-mutated form, significantly increasing the risk of deep vein thrombosis and pulmonary embolism.

Intended Use: Imegen-Cambridge II is strictly intended for research purposes and is tailored for use by molecular biology professionals.

Kit Contents and Storage Conditions: The Imegen-Cambridge II kit includes all necessary reagents for 48 determinations, divided as follows:

• Cambridge II Master Mix (Red Cap): 2 x 180 µL, store at -20ºC
• General Master Mix (White Cap): 2 x 300 µL, store at 4ºC

Unlock the power of Imegen-Cambridge II to advance your research in the field of genetics and gain valuable insights into Cambridge II Syndrome-associated genetic mutations