Enterovirus PCR Kit

Suitable for using as primary screening method

An enterovirus infection is a collection of different types of infections caused by enteroviruses. Enteroviruses is a collective name for several viruses including polioviruses, echoviruses, coxsackieviruses. Enterovirus is a virus that is very common and can be easily transmitted. Newborn babies up to 6 weeks of age and young adults are most susceptible to Enterovirus. When infected, symptoms can occur such as fever, red spots, diarrhoea, vomiting and inflamed eyes. In most cases, automatic recovery of the infection takes place after 3 to 4 days.

Viral RNA is detected during the detection of the enterovirus. For such a detection, collection material is taken, which may be faeces, throat swab, vesicle swab, rinse and liqour. Presence of virus exclusively in the throat and faeces is often not evidence of a recent infection.

During the PCR detection of enterovirus, amplification takes place of the 5’UTR RNA sequence which serves as the target sequence. The 5’UTR sequence is composed of stably folded RNA domains. Proper folding and functioning of the 5′-UTR underlies the efficiency of viral replication and also determines viral virulence. By detecting the 5’UTR RNA, it is possible to analyze whether the enterovirus is present.

PRODUCT ADVANTAGES

COMPLIANCE WITH ENPEN REQUIREMENTS
Amplification of a single-copy 5‘ UTR RNA sequence which is required as the target sequence by European non-polio enterovirus network (ENPEN) Suitable for using as primary screening method
DETECTION OF ALL HUMAN ENTEROVIRUS SPECIES
The kit enables detection of Enterovirus species A-D including Coxsackievirus, Echovirus, Enterovirus, Poliovirus and the newly discovered Enterovirus group EV-C104 with nucleotide changes in 5´-UTR sequence. The kit does not include Rhinovirus detection
QUALITATIVE AND SEMIQUANTITATIVE DETECTION
Monitoring of pathogen level in time
CONTAMINATION PREVENTION
Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating carryover contamination
COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES
CE IVD CERTIFIED DIAGNOSTIC TEST