HIV type 1 Diagnostic PCR kit

Protection agains detectino failures caused by virus mutations

HIV stands for Human Immunodeficiency Virus and belongs to the group of retroviruses. When infected with the HIV-1 virus, the virus attacks the immune system by destroying CD4 cells, or the white blood cells that are crucial to fight infection. This destruction makes the body susceptible to life-threatening conditions, such as infections or cancer. HIV belongs to the retrovirus family, a characteristic of which is that there is a long period between an infection and the first symptoms of disease. During an infection with HIV-1, especially the lymph nodes, spleen and thymus are damaged by spreading through the blood, this is called the acute phase. Subsequently, the immune system can recover, so that HIV-1 remains latent in the virus and does not cause any symptoms, HIV can only affect organs. Long-term latent HIV-1 infection can lead to AIDS. The disease AIDS can weaken the immune system to such an extent that life-threatening opportunistic infections can develop.

With the HIV type 1 PCR kit HIV-1 can be detected by means of a PCR analysis. HIV-1 encodes the polypeptide GaG which is capable of forming virus-like particles in vitro in the absence of cellular or viral components. The HIV type 1 PCR kit can detect the polypeptide Gag. Also, this PCR kit can detect the LTR sequence when analyzed for HIV-1. LTR is a transposable element characterized by the presence of long terminal repeats flanking an internal coding region. LTRs mobilize through reverse transcription of their mRNA and integration of cDNA at a different location. The difference between this PCR kit and the other HIV-1 PCR kit is the fact that besides Gag gene and the LTR sequence this PCR kit also focuses on the pol gene which is something the other PCR kit doesn’t. The pol gene has an interaction with the gag gene which causes a expression for HIV-1 analyses with this kit.

PRODUCT ADVANTAGES

DUAL TARGET DETECTION
Protection against detection failures caused by virus mutations
QUALITATIVE AND QUANTITATIVE DETECTION
Monitoring of pathogen level in time. Full traceability to WHO standard.
EASY-TO-USE CONCEPT
Single tube Ready-to-Use Master Mix contains all components for PCR amplification. No additional PCR reagents pipetting necessary.
CONTAMINATION PREVENTION
Master Mix contains Uracil-DNA glycosylae and dUTPs eliminating carryover contamination

COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES

CE IVD CERTIFIED DIAGNOSTIC TEST