Human herpes virus 8 PCR kit

Dual targeting prevents a detection failure caused by the occurrence of mutations

>Human Herpes Virus 8 is etiologically linked to all forms of Kapos (KS), including, endemic, transplant-related and AIDS-related. A patient infected with an HHV-8 infection is at risk of developing KS, as well as an infection with HHV-8 with multicentric Castleman disease. HHV-8 is mainly transmitted through the word. As an infected KS, enlargement is involved as the appearance of brownish and bluish nodules that tended to appear in dome-shaped tumors. played nodules were found in the intestines, stomach, liver, trachea and in the larynx. When the tumors enlarge and expand, cancer can develop.

HHV-8 has genes in its genome that are very similar to human genes, which means that these genes are probably of human origin. This makes it possible for the virus to evade or block any proteins of the immune system and thus cause tumors.

Diagnosis of HHV-8 can be made by immunohistochemical staining of tumors with antibodies that recognize the HHV-8 encoded latency-associated nuclear antigen, LANA. To diagnose HHV-8, the PCR kit is used, for which EDTA plasma is essential as host source material to detect HHV-8. Also, during the detection of HHV-8 by this PCR kit, several genes are analyzed which serve as target sequence. These are the genes ORF26 and ORF73. The ORF26 gene encodes a cytoplasmic protein whose expression is induced 16-fold by treatment with sodium butyrate. This protein induction correlates with a significant induction of viral RNA transcripts, leading to an increase in viral DNA. the ORF73 gene encodes LANA. LANA is identified in HHV-8 infected cell lines as previously described by an immunofluorescence assay.

PRODUCT ADVANTAGES

DUAL TARGET DETECTION
Dual targeting prevents a detection failure caused by the occurrence of mutations
SIMPLE LABORATORY WORKFLOW
Easily combinable with other GeneProof PCR kits in one workflow
QUALITATIVE AND QUANTITATIVE DETECTION
Unique possibility to quantify viral load
EASY-TO-USE CONCEPT
Single tube Ready-to-Use Master Mix contains all components for PCR amplification. No additional PCR reagents pipetting necessary.
CONTAMINATION PREVENTION
Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating carryover contamination

COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES
CE IVD CERTIFED DIAGNOSTIC TEST