MH/UU/UP Multiplex PCR Kit

PCR Kit detects and distinguishes the important STI pathogens in a single reaction tube:

This product diagnoses 3 pathogens from one sample in a run, diagnostics of Mycoplasma hominis, Ureaplasma urealyticum and ureaplasma parvum are performed. The PCR diagnosis focuses on the ureD gene for u. urealyticum and u. parvum and on the gap gene for m. hominis. All three pathogens belong to the Mollicutes class and the Mycoplasmataceae family of bacteria.

The ureD gene is a gene that codes for the accessory proteins. This gene is also necessary for the urease activity, it is resistant to EDTA and sodium citrate and this gene is sensitive to flurofamide. Due to the urease activity of the gene it forms the main component of the cytoplasm and is composed of several 70 kilo-kilodelton polypeptides.

The gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). This gene is expressed in mycoplasma hominis, detection of this expression during PCR diagnosis can determine whether the host is infected with humanoids.

Ureaplasma urealyticum belongs to the group of genital mycoplasma organisms, ureaplasma can cause a urethritis that is sexually transmitted. Ureaplasma is parasitic, meaning it needs a host, such as humans or animals, to survive. Infected persons do not have to suffer from complaints when ureplasma is in balance with other bacteria. Ureaplasma parvum is a descendant of ureaplasma urealyticum, so there are few variations between these two bacteria. These pathogens are distinguished from mycoplasma hominis in that they have the ability to hydrolyze urea for energy production.

Mycoplasma hominis is a bacterium that has the ability to penetrate into the interior of human cells. This bacterium is the cause of inflammation in the pelvis and bacterial vaginosis, the bacterium can also lead to infertility in both women and men..

PRODUCT ADVANTAGES

MORE INFORMATION FROM ONE REACTION
PCR Kit detects and distinguishes the important STI pathogens in a single reaction tube: Mycoplasma hominis Ureaplasma urealyticum Ureaplasma parvum
SIMPLE LABORATORY WORKFLOW
Easily combinable with other GeneProof PCR kits in one workflow
CONTAMINATION PREVENTION
Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating carryover contamination
EASY-TO-USE CONCEPT
Single tube ready-to-use Master Mix contains all components for PCR amplification No additional PCR reagents pipetting necessary
COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES
CE IVD CERTIFIED DIAGNOSTIC TEST