Molysis Basic 5 Kit
MolYsis Basic5 is a sample pre-treatment kit for background-free PCR on whole blood and other body fluids. It lyses the human cells, degrades the released human DNA and degrades the cell walls of the microbial cells that remain. Research use only. The DNA isolation itself is done with a separate downstream kit, spin column or automated system of the laboratory’s choice. Four parallel pre-treatments take about 80 minutes, of which 15 minutes are hands-on.
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This kit in detail
- Pre-treatment only; DNA isolation with a kit of your own choice
- Human DNA degraded by the chaotrope-resistant DNase MolDNase B
- About 80 minutes for four pre-treatments, 15 minutes hands-on
- Protocols for samples up to 1 ml and for 5 ml
- Whole blood on EDTA or citrate, and other body fluids
- 50 or 100 reactions per kit, REF D-301-050 and D-301-100
MolYsis Basic5 prepares a sample so that a subsequent PCR is not dominated by human DNA. It is the pre-treatment step on its own, for laboratories that already have their own DNA isolation in place.
Principle
A chaotropic buffer is added to the sample, which lyses the human or animal cells while the pathogens stay intact. The nucleic acids released are degraded by MolDNase B. The microbial cells are then collected by centrifugation and their cell walls degraded with BugLysis. The lysate goes into the DNA isolation method of the laboratory: a separate kit, mini spin columns or an automated system.
Sample material
Whole blood stabilised with EDTA or citrate, synovial fluid, pleural fluid, cerebrospinal fluid, ascites, pus, bronchoalveolar lavage, nasal douche and urine. There is a protocol for samples up to 1 ml and one for 5 ml. Frozen samples without a cryoprotectant are not suitable.
Practical points
Four parallel pre-treatments take about 80 minutes, including 15 minutes of hands-on time. The buffers are stored at room temperature and MolDNase B, BugLysis and beta-mercaptoethanol frozen. No controls are supplied. If DNA isolation in the same kit is preferred, the manufacturer offers MolYsis Complete5.
Regulatory status
This kit is for research use only and not for use in diagnostic procedures.
- Lysis Buffer: A solution containing chaotropic salts and detergents that disrupt the cell membranes and release the microbial DNA into solution.
- Proteinase K: An enzyme that degrades proteins, including nucleases and proteases that could degrade the extracted DNA.
- Lysis Enhancer: A solution that enhances the lysis of microbial cells and facilitates the release of DNA into solution.
- Carrier RNA: RNA molecules that act as a carrier for the DNA during the extraction process and improve DNA recovery and yield.
- DNase/RNase-free Water: Water that has been treated to remove contaminating DNases and RNases, which can degrade the extracted DNA.
The kit also includes instructions for use, as well as a protocol for the extraction of DNA from different sample types, such as blood, urine, or tissue.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Molysis Basic 5 Kit.
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