The MycoGENIE® Aspergillus fumigatus and resistances TR34/L98H Real Time PCR kit is a qualitative quadruplex PCR that targets 28s rDNA from Aspergillus fumigatus and TR34/L98H mutations. This kit has been validated on serum, plasma, sputum, BAL, fresh and frozen tissues. It has an analytical sensitivity of 6 copies for the monocopy genes (mutations TR34/L98H) and 1 copy for the multicopy gene (Aspergillus fumigatus rDNA).
The kit has been validated on : Roche Diagnostics® (LightCycler® 480 real-time instrument I & II) BioRad (CFX96 real-time PCR detection system), Applied Biosystems® (ABI7500 real-time PCR system), Cepheid® (SmartCycler II®), Agilent technologies (MX3000P qPCR system), Qiagen (Rotorgene).