The neisseria ghonorroeae PCR kit doubles detection of the 16s rRNA gene and the porA pseudogene. The bacterium neisseria ghonorroeae expresses the outer membrane porin, porA pseudogene. The sequence of this gene manifests itself in 36 sequence variants of which 30 have not been previously described, making the porA pseudogene a suitable genetic target for the detection of Neisseria ghonorroeae. This sequence consists of a 76 bp fragment and the 5′ untranslated region of the porA pseudogene.

During detection, the PCR kit uses two probes, of which one of the two probes recognizes a newly identified sequence in the grandpa genes. Sample material used for the PCR diagnosis is semen, swab and urine. There can be a difference in a PCR diagnosis in men or women. A urethral smear is preferred for men, while a cervical smear is preferred for women.