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The beta (β)-globin strip assay from Viennalab is a molecular diagnostic test designed to detect genetic variants associated with hemoglobinopathies such as sickle cell anemia and beta-thalassemia. This assay is a fast and reliable tool for identifying patients who may carry these mutations, allowing for earlier diagnosis and appropriate treatment.
The β-globin strip assay utilizes a reverse hybridization method, in which PCR amplification is followed by hybridization of the biotin-labeled PCR product to specific probes immobilized on a nitrocellulose membrane strip. This allows for the detection of various beta-globin gene mutations through the appearance of specific hybridization signals on the strip.
The β-globin strip assay is a qualitative test that can detect various beta-globin gene mutations, including the most common mutations associated with sickle cell anemia and beta-thalassemia. The test is designed to detect up to 10 different mutations in a single reaction, providing a comprehensive diagnostic tool for patients with hemoglobinopathies.
One of the key advantages of the β-globin strip assay is its high sensitivity and specificity. The assay has been shown to have a sensitivity of 99% and a specificity of 100%, making it a highly reliable tool for identifying patients with hemoglobinopathies. Additionally, the assay is easy to use and does not require specialized equipment, making it accessible for laboratories with limited resources.
The β-globin strip assay can be used for both research and clinical applications, providing a valuable tool for genetic counseling, carrier screening, and prenatal diagnosis. The test can be performed on a variety of sample types, including whole blood, dried blood spots, and buccal swabs, making it a flexible and versatile tool for genetic testing.
In summary, the β-globin strip assay from Viennalab is a fast, reliable, and easy-to-use molecular diagnostic tool for identifying genetic variants associated with hemoglobinopathies. The assay’s high sensitivity and specificity, along with its ability to detect multiple mutations in a single reaction, make it a valuable tool for both research and clinical applications.