Introduction:

DPYD PharmaKitDx is a genetic testing solution that identifies patients with dihydropyrimidine dehydrogenase (DPD) deficiency. DPD is an essential enzyme responsible for metabolizing fluoropyrimidine drugs used in cancer chemotherapy, like 5-fluorouracil (5-FU) and capecitabine.

Understanding DPD Deficiency :

Certain individuals carry genetic variations in the DPYD gene, leading to reduced or absent DPD activity. This condition puts them at a higher risk of severe and potentially life-threatening side effects when receiving standard doses of fluoropyrimidine medications. Early detection of DPD deficiency allows healthcare professionals to tailor treatments and make informed decisions for each patient’s optimal outcomes.

Simple Process for Researchers and Lab Technicians:

Using DPYD PharmaKitDx is straightforward for researchers and lab technicians. The kit provides all necessary materials and detailed instructions to collect a patient’s DNA sample, usually through blood or saliva. Within a short time, the lab delivers comprehensive genetic testing results, indicating the patient’s DPD status. Based on these results, clinicians can customize the treatment plan for maximum safety and efficacy.

The Future of Personalized Medicine:

DPYD PharmaKitDx represents a significant stride towards personalized medicine in oncology. As pharmacogenomics advances, similar genetic testing kits may emerge for other drugs and conditions, further enhancing patient outcomes and improving quality of life.

Specifications:

• Amount of DNA needed: 50 ng total
• Type of sample: Peripheral blood (Germinal)
• Number of reactions per sample: 4
• Number of targets: 4
• Manual work time: 15 min
• Duration of the PCR program: 1h 30 min
• Compatible cyclers: real-time PCR cycler with FAMTM and VICTM channel
• Qualitative analysis that allows the detection of DPYD alleles:
  • DPYD*2A [NM_000110.4: c.1905+1G>A (rs3918290)]
  • DPYD*13 [NM_000110.4: c.1679T>G (rs55886062)]
  • DPYD*hapB3 [NM_000110.4: c.1129-5923C>G (rs75017182)]
  • DPYD-D949V [NM_000110.4: c.2846A>T (rs67376798)