Factor II dPCR kit
The Imegen Factor II dPCR kit determines the genotype of the prothrombin variant c.*97G>A, also written 20210G>A, in the F2 gene by digital PCR with allele-specific hydrolysis probes. Research use only. The variant raises the prothrombin concentration in plasma and is, after Factor V Leiden, the second most frequent hereditary risk factor for venous thromboembolism. One kit covers 48 reactions.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Determines the F2 c.*97G>A (20210G>A) genotype
- Digital PCR with allele discrimination by VIC and FAM probes
- Factor II Control and a no template control supplied
- QuantStudio 3D and Bio-Rad digital PCR platforms
- 48 reactions per kit, REF IMG-331
The Imegen Factor II dPCR kit determines the genotype of the prothrombin variant c.*97G>A in the F2 gene, the variant usually written as 20210G>A.
Clinical background
The variant lies in the 3′ untranslated region of the prothrombin gene and raises the prothrombin concentration in plasma. Carriers have an increased risk of venous thromboembolism, and that risk rises further in combination with Factor V Leiden or with acquired factors such as immobilisation, surgery, pregnancy or oral contraceptive use. The genotype is determined as part of a thrombophilia work-up.
Method
Digital PCR with two allele-specific hydrolysis probes, labelled with VIC and FAM, discriminates the wild-type and variant allele. The kit contains the Factor II Master Mix in four vials of 12 reactions, the Factor II Control and a no template control. Two thermal cycling programmes are given, one for the QuantStudio 3D and one for the Bio-Rad platform.
What the manual does not state
The manufacturer’s manual for this kit gives no intended-use statement, no accepted sample material, no DNA input and no sensitivity data. Those figures are therefore not claimed here.
Regulatory status
This kit is for research use only.
The kit is equipped with reagents for 48 reactions, including:
- Factor II Master Mix: Lyophilised master mix containing the oligonucleotides and hydrolysis
probes for the multiplexed amplification of the wild type and the mutated genotype associated
to prothrombin. F2 wild type and F2 mutated genotype are labelled with fluorophores VIC® and
FAM™, respectively. - Factor II Control: Aqueous positive control is heterozygous for the G-to-A transition at position
20210 of the prothrombin gene (20210G>A).
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Factor II dPCR
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Factor II dPCR kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
