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SepsiTest™-UMD Kit

Short description

The SepsiTest UMD is an in-vitro diagnostic kit that offers a rapid, unbiased, and culture-independent pathogen detection and identification of bacterial and fungal pathogens in various clinical specimens. The test can detect non-culturable and antibiotic-inhibited pathogens and identify more than 1,350 microorganisms.

The kit comes with one protocol suitable for a broad range of clinical specimens, including fluids, tissues, and swabs. The test uses PCR or Real-Time PCR assays targeting 16S and 18S rRNA genes of bacteria and fungi and allows for the identification of bacterial and fungal species by Sanger sequencing.

The kit is suitable for daily routine diagnosis of materials that often remain negative in culture due to fastidious pathogens or antibiotic treatment before sampling. The kit can be used with whole blood, CSF, BAL, synovial aspirates, ascites fluid, peritoneal aspirates, pus, blood culture, ultrasonic fluids (prostheses), tissue biopsies, and swabs from various sources. The process of microbial DNA enrichment and isolation from body fluids and tissue samples is also available semi- and fully automated.

Product highlights

  • Broad range of bacteria and fungi
  • Detects intact, non-culturable, and antibiotic-inhibited pathogens
  • More than 1,350 microorganisms identified
  • PCR or Real-Time PCR assays targeting 16S and 18S
  • All reagents guaranteed DNA-free
  • Ideal for daily routine diagnosis

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SepsiTest™-UMD Kit

Specifications of the SepsiTest™-UMD Kit

SepsiTest™-UMD from Molzym is the ultimate solution for rapid and accurate pathogen detection and identification of bacterial and fungal pathogens in a wide range of clinical specimens including body fluids, tissues, and swabs. With a simple protocol for all types of specimens, the test detects intact, non-culturable, and antibiotic-inhibited pathogens, making it ideal for the identification of fastidious pathogens.

With more than 1,350 microorganisms identified so far, the test boasts of high diagnostic sensitivity and specificity, making it the perfect tool for daily routine diagnosis of specimens that often remain negative in culture. The CE IVD-marked molecular test is designed for the culture-independent detection and identification of a broad range of Gram-positive bacteria, Gram-negative bacteria, and fungi within a working day.

SepsiTest™-UMD comes with PCR or Real-Time PCR assays targeting 16S and 18S rRNA genes of bacteria and fungi, identification of bacterial and fungal species by Sanger sequencing, PCR and extraction controls, and reagents guaranteed to be DNA-free. The test can analyze up to 10 ml samples in combination with Add-On 10.

Typical specimens that can be tested with SepsiTest™-UMD include whole blood, CSF, BAL, synovial aspirates, ascites fluid, peritoneal aspirates, pus, blood culture, ultrasonic fluids (prostheses), tissues biopsies, including abscesses, bones, organs, heart valves, stents, pacemakers, and swabs from wounds, organs, bones, prostheses, teeth, and others.

With a sequencing strategy and BLAST analysis and an option for semi- and fully automated testing, SepsiTest™-UMD offers the ultimate solution for rapid and accurate detection and identification of bacterial and fungal pathogens in a wide range of clinical specimens.

Components of the SepsiTest™-UMD Kit
  1. Lysis Buffer: A solution containing chaotropic salts and detergents that disrupt the red blood cells and release the microbial DNA into solution.
  2. Magnetic Beads: Beads coated with specific oligonucleotide probes that capture and isolate microbial DNA from the sample.
  3. Wash Buffer: A solution used to wash the magnetic beads and remove any remaining contaminants.
  4. Elution Buffer: A solution used to release the captured microbial DNA from the magnetic beads.
  5. Control DNA: A synthetic DNA that is added to the sample as an internal control to ensure that the assay is working correctly.
  6. PCR Master Mix: A solution containing all of the reagents necessary for PCR amplification of the captured microbial DNA.
  7. DNA Size Marker: A DNA ladder used to determine the size of the amplified PCR products.
  8. PCR Primers: Specific primers that amplify conserved regions of the microbial DNA for detection.
  9. PCR Enzyme: A heat-stable DNA polymerase enzyme used to catalyze the PCR reaction.
  10. DNase/RNase-free Water: Water that has been treated to remove contaminating DNases and RNases, which can degrade the extracted DNA.

 

The kit also includes instructions for use, as well as a protocol for the detection of microbial DNA in blood samples using real-time PCR.

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