FXIII V34L PCR Assay
The Viennalab FXIII V34L RealFast Assay detects the G>T transition in exon 2 of the F13A gene by real-time PCR and discriminates the three genotypes VV, VL and LL in a human genomic DNA extract. The variant replaces valine by leucine at position 34 of the A subunit of coagulation factor XIII, the enzyme that cross-links fibrin. The L allele is activated faster by thrombin and is described as protective against venous thrombosis and myocardial infarction, which makes the genotype relevant in a thrombophilia work-up.
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This kit in detail
- Detects F13A V34L (g.7130G>T, rs5985)
- Discriminates the VV, VL and LL genotypes
- Real-time PCR with allele-specific hydrolysis probes, 114 bp amplicon
- WT-Control and MUT-Control supplied
- Whole peripheral blood, dried blood spots, buccal swabs or saliva
- 32 or 100 reactions per kit, REF 7-253 and REF 7-250
The Viennalab FXIII V34L RealFast Assay determines the genotype of the V34L variant in the gene for the A subunit of coagulation factor XIII.
Clinical background
Factor XIII cross-links fibrin monomers and so gives the clot its mechanical strength and its resistance to fibrinolysis. The V34L variant lies close to the thrombin cleavage site, so the L allele is activated faster. The variant is described in the literature as protective against venous thrombosis and myocardial infarction, and is therefore determined as part of a thrombophilia work-up rather than on its own.
Method
The assay is a real-time PCR based on the 5′ nuclease principle. A 114 bp fragment of the F13A gene is amplified and read with two allele-specific hydrolysis probes: HEX for the V allele and FAM for the L allele, so the result is VV, VL or LL. The kit supplies the RealFast 2x Genotyping Mix, the FXIII V34L Assay Mix, a WT-Control and a MUT-Control. The recommended DNA input is 10 to 100 ng per reaction, at a concentration of 2 to 20 ng/µl, and the limit of detection is 0.2 ng of genomic DNA per reaction. DNA isolated from whole peripheral blood, dried blood spots, buccal swabs or saliva can be used, and DNA extraction reagents are not supplied with the kit.
- RealFast™ 2x Genotyping Mix
- FXIII V34L Assay Mix
- FXIII V34L WT-Control
- FXIII V34L MUT-Control
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the FXIII V34L PCR Assay.
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