Molzym’s Mastermix 18S Complete is a highly purified and optimized master mix that is specifically designed for the amplification of eukaryotic 18S rRNA genes. The master mix contains all the necessary components for PCR amplification, including a highly sensitive and specific Taq DNA polymerase enzyme, optimized buffer, dNTPs, and additives for PCR optimization.
The Taq DNA polymerase enzyme in Molzym’s Mastermix 18S Complete is highly purified and has a high processivity, which enables it to amplify DNA fragments up to 5 kb in length. Additionally, the enzyme has a low error rate, which means that it is highly reliable in PCR experiments. The optimized buffer in the master mix provides optimal conditions for the enzyme activity, ensuring efficient and specific amplification of eukaryotic 18S rRNA genes.
There are several advantages of using Molzym’s Mastermix 18S Complete in PCR experiments. Firstly, the master mix contains all the necessary components for PCR amplification, which saves time and reduces the risk of contamination during preparation. Secondly, the optimized master mix is highly sensitive and specific, which means that it can detect low amounts of eukaryotic DNA in a sample.
Another advantage of Molzym’s Mastermix 18S Complete is its versatility in PCR amplification of eukaryotic 18S rRNA genes from a variety of sources, including clinical and environmental samples. The master mix is compatible with different PCR conditions, making it easy to use in different experimental setups.
Molzym’s Mastermix 18S Complete has several applications in molecular biology research. It is commonly used for the detection and identification of eukaryotic pathogens in clinical and environmental samples. The master mix is also used for microbial diversity studies, where it is used to amplify eukaryotic 18S rRNA genes for sequencing or cloning.