Presto 100 CT/NG PCR Kit, tap a thumbnail to see more views.
Presto 100 CT/NG PCR Kit
The Presto 100 CT/NG PCR Kit detects Chlamydia trachomatis and Neisseria gonorrhoeae by multiplex real-time PCR. The assay amplifies the CT cryptic plasmid and the NG opa genes, and detects both in the same reaction, so a double infection is identified in a single test. An isolation amplification control is included to reveal inhibition or failed isolation. One kit covers 100 reactions.
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This kit in detail
- Chlamydia trachomatis and Neisseria gonorrhoeae in one reaction
- Targets the CT cryptic plasmid and the NG opa genes
- Double infection detected in a single test
- Isolation amplification control for inhibition and isolation failure
- Separate positive controls for CT and for NG, plus a negative control
- 100 reactions per kit, made in the Netherlands
The Presto 100 CT/NG PCR Kit detects Chlamydia trachomatis and Neisseria gonorrhoeae, the two most frequently diagnosed bacterial sexually transmitted infections, by multiplex real-time PCR.
Targets and detection
The assay amplifies a specific segment of the CT cryptic plasmid and of the NG opa genes. The amplification products are detected in real time with internal probes, each carrying a separate fluorescent reporter dye, so both organisms are reported from the same reaction and a double infection is identified in one test.
Isolation amplification control
An isolation amplification control is carried through the procedure. It shows whether inhibitory substances in the clinical sample interfered with amplification, or whether isolation failed, which is what distinguishes a true negative from a failed run.
Controls and format
The kit contains a ready-to-use PCR mix, the isolation amplification control, separate positive controls for CT and for NG, a negative control, and a CT and an NG selector. One kit covers 100 reactions and is made in the Netherlands.
CT/NG PCR mix MASTER MIX (CG301001). 4 Tubes with a black colour code, labelled ”MASTER MIX” containing 400 µl ready to use PCR mix. This mixture contains four primers for amplification of the CT DNA, NG DNA and the Isolation Amplification Control DNA. It contains three fluorescent probes for detection of CT DNA, NG DNA and control DNA respectively. The master mix contains all ingredients for PCR amplification including DNA polymerase.
CT/NG Isolation Amplification Control Isolation Amplification Control (CG301003). 4 Tubes with a red colour code, labelled ”Isolation Amplification Control” containing 150 µl Isolation Amplification Control. The Isolation Amplification Control consists of an inactivated E. coli modified with a genomic DNA fragment containing primer binding sites identical to the C. trachomatis and N. gonorrhoeae sequences, but with a different intermediate probe sequence. NOTE: resuspend before use.
CT Positive Control Positive Control CT (CG301005). 4 Tubes with a blue colour code, labelled “Positive Control CT” containing 55 µl positive control. The positive control consists of CT DNA at 4 IFU/ 10 µl.
NG Positive Control Positive Control NG (CG301006)
4 tubes with a green colour code, labelled ”Positive Control NG” containing 55 µl positive control. The positive control consists of NG DNA at 100 CFU/ 10 µl.
CT/NG Negative Control Negative Control (CG301014). 2 Tubes with a transparent colour code, labelled ”Negative Control” containing 500 µl negative control.
CT Selector CT SELECTOR (CG301008). 1 Tube with a violet colour code, labelled ”CT SELECTOR” containing 50 µl of CT selector for use with high NG positive samples for the detection of a weak positive CT-co-infection.
NG Selector NG SELECTOR (CG301007). 1 Tube with a yellow colour code, labelled ‘NG SELECTOR’ containing 50 µl of NG selector for use with high CT positive samples for the detection of a weak positive NG-co-infection.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Publications
This kit, put to work
Protocol detail and workflow guidance from labs already running this kit, straight from our application notes library.
All application notesFrequently paired with this kit
Other assays and instruments lab teams add alongside the Presto 100 CT/NG PCR Kit.
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