With the VZV PCR kit, both an acute infection can be detected, but also an infection that has already occurred before, an indirect infection. The presence of virus-specific IgG antibodies in the serum indicates a past infection. In most cases, VZV leading to chickenpox and/or shingles is a clinical or visual diagnosis. When in doubt, a detection by PCR is applied. PCR detection then takes place on vesicle fluid from a skin vesicle. This is the most sensitive method to detect an infection of VZV. In this VZV PCR kit, an analysis is performed on target sequence of the gene ORF62. This gene is a virion-associated transactivator. Expression of ORF62 increases the infectivity of VZV DNA. This means that ORF62 plays a critical role in initiating infection because high expression increases the likelihood that transfected VZV DNA will result in a productive infection.