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The TRUPCR® Toxoplasma Detection Kit is a highly sensitive and specific real-time PCR solution designed for the qualitative detection of Toxoplasma gondii DNA in human serum or plasma samples. Toxoplasma infections, while often mild or asymptomatic, can lead to severe complications in immunocompromised patients and during pregnancy. This kit enables laboratories to rapidly and reliably identify Toxoplasma-positive samples, ensuring early diagnosis and improved patient outcomes.
Each reaction is multiplexed to include two parallel targets: one for Toxoplasma-specific DNA and another for the endogenous internal control (IC) to validate sample integrity and rule out PCR inhibition. The assay uses hot-start technology to prevent non-specific amplification and ensures optimal performance in terms of both accuracy and reproducibility.
With compatibility across major real-time PCR platforms (ABI, Bio-Rad, Roche, Qiagen), a streamlined workflow, and flexible kit sizes, this solution is ideal for clinical diagnostic labs performing routine or high-throughput Toxoplasma-targeted testing.
The TRUPCR® Toxoplasma Detection Kit is a molecular diagnostic test for the qualitative detection of Toxoplasma gondii, the protozoan parasite responsible for toxoplasmosis. The assay targets highly conserved genomic sequences unique to Toxoplasma gondii, ensuring accurate differentiation from other pathogens.
The kit is designed as a multiplex real-time PCR assay with two reactions per tube:
One target detects Toxoplasma gondii DNA in the HEX/VIC/Yellow channel
The second monitors the internal control DNA in the TEX-RED/ROX/Orange channel
It is validated for use with serum and plasma samples, supporting routine testing in clinical laboratories dealing with infectious disease diagnostics and prenatal screenings.
Toxoplasma gondii is a ubiquitous protozoan parasite, with cats as definitive hosts but capable of infecting nearly all warm-blooded animals, including humans. While Toxoplasma-positive infections are often asymptomatic in immunocompetent individuals, they can cause serious complications under certain conditions.
In pregnant women, a primary Toxoplasma infection can cross the placenta, potentially resulting in congenital toxoplasmosis. This may lead to hydrocephalus, chorioretinitis, or even intrauterine death. For immunocompromised individuals—such as those with HIV/AIDS or organ transplant recipients—latent infections can reactivate, leading to life-threatening conditions like toxoplasmic encephalitis.
Timely and accurate diagnosis of Toxoplasma-targeted infections is vital to prevent irreversible outcomes. Molecular testing using real-time PCR is the gold standard for early, sensitive detection.
The TRUPCR® Toxoplasma Detection Kit is an in vitro diagnostic (IVD) real-time PCR assay developed to meet the high sensitivity and specificity demands of modern clinical laboratories. It features a multiplex reaction format that enables simultaneous amplification of Toxoplasma gondii DNA and an endogenous internal control.
Key product characteristics:
Assay Type: Qualitative detection
Target Pathogen: Toxoplasma gondii
Validated Specimens: Human serum or plasma
Control: Internal control DNA included in each reaction
Storage: Compatible with standard lab conditions
The kit is available in multiple formats and extraction-compatible options to suit the needs of both low- and high-throughput labs.
This real-time PCR assay uses a fluorophore-quencher probe system for amplification detection. The test follows these main steps:
DNA Extraction: From serum or plasma using validated methods
Reaction Setup: Add DNA and reagents into a single-tube multiplex PCR reaction
PCR Amplification:
Toxoplasma DNA detected in HEX/VIC/Yellow channel
Internal control DNA in TEX-RED/ROX/Orange channel
Real-Time Monitoring: Fluorescence signals increase proportionally with DNA amplification
The assay uses hot-start Taq polymerase to minimize background noise and improve amplification efficiency. Results are interpreted using threshold cycle (Ct) values to determine the presence or absence of Toxoplasma DNA.
This dual-target system ensures reliable detection and eliminates false negatives caused by PCR inhibitors or poor sample quality.
The TRUPCR® Toxoplasma Detection Kit is an essential tool for:
Prenatal screening for congenital toxoplasmosis
Diagnosis in immunocompromised patients (HIV, transplant, oncology)
Routine serological confirmation of suspected acute or latent infections
Research labs studying Toxoplasma epidemiology or pathogenesis
The ability to detect Toxoplasma early and accurately reduces patient risk, informs therapeutic decisions, and contributes to overall public health strategies.
Because the parasite can remain latent for years and reactivate under immunosuppression, routine Toxoplasma-targeted PCR testing is increasingly integrated into hospital protocols, particularly in neurology, transplant medicine, and obstetrics.
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