The Molzym Mastermix 16S Complete is a ready-to-use PCR reagent that contains all the necessary components for the amplification of bacterial DNA, except for the template and DNA-free water. The mastermix is designed to target conserved regions of the 16S rRNA gene, which is present in all bacteria. As such, it can amplify any eubacterial DNA, regardless of species or strain.
The kit comes in a 2.5x-concentrated solution, allowing for high-throughput bacterial DNA amplification. It can produce an amplicon of approximately 450 bp, which contains variable sequences for the identification of bacteria by taxon-specific probing or sequence analysis. The amplified DNA can be detected using several methods, including gel electrophoresis, Real-Time PCR with intercalating fluorescent dyes or probes, or array technologies.
One of the major advantages of the Molzym Mastermix 16S Complete is its efficiency in detecting low levels of bacterial DNA. The kit uses a proprietary technology that selectively removes human DNA from the sample, thereby reducing background noise and increasing sensitivity. As a result, the kit can detect bacterial DNA in a variety of sample types, including blood, urine, cerebrospinal fluid, and tissue samples, among others.
Another advantage of the Molzym Mastermix 16S Complete is its versatility. The kit is suitable for a wide range of applications, including diagnosis of bacterial infections, monitoring of bacterial contamination in food and water, and microbiome analysis. The mastermix is also compatible with different detection methods, allowing for flexibility in experimental design.
The Molzym Mastermix 16S Complete is easy to use, and its simple protocol can be completed in a relatively short time. The kit also includes components such as SYBR Green 1 DNA staining solution for visualization, DNA marker for size estimation of the amplicon, and gel loading solution, making it a comprehensive solution for bacterial DNA amplification.
In conclusion, the Molzym Mastermix 16S Complete is an efficient, versatile, and sensitive tool that laboratory professionals and scientists can use for bacterial DNA amplification, detection, and identification. Its unique technology, coupled with its high-throughput capabilities, make it an excellent choice for various applications, from clinical diagnostics to environmental monitoring.