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Aspergillus is a genus of fungi that can cause a range of diseases, especially in immunocompromised individuals. Commonly found in the environment, Aspergillus spores can be inhaled and lead to infections like aspergillosis. These infections can range from allergic reactions to more severe conditions affecting the lungs or even disseminating to other parts of the body. In the diagnostic setting, early and accurate detection of Aspergillus is crucial. Various diagnostic tests, including culture methods and molecular techniques, are employed to identify the presence of the fungus, enabling timely medical intervention and treatment.