The BIOSYNEX AMPLIQUICK® Fecal Pretreatment kit is a manual kit for pretreating fecal samples to screen for intestinal parasites using gene amplification.
The kit comes in pre-filled vials with ready-to-use grinding beads and pretreatment buffer, designed to release nucleic acids from the biological samples. It is intended for use in molecular diagnostics only and is validated for use with the BIOSYNEX AMPLIQUICK® Helminths and Protozoans PCR kits. The kit optimizes the release of DNA from biological samples for molecular detection testing and is based on the physico-chemical lysis of the cell membrane components or the wall of parasites, cystic forms or eggs.
The kit’s lysis stages are optimized to increase the ratio of non-human DNA extracted. After centrifugation, the DNA remains suspended in the supernatant and can be extracted using a suitable kit.
The kit also includes instructions for use, as well as a protocol for the extraction of genomic DNA from fecal samples using a magnetic bead-based method.