What is in the Dengue Detection Kit?

The TRUPCR® Dengue Detection Kit (V-1.2) is an in vitro nucleic acid amplification test for the qualitative detection of Dengue virus (DENV) RNA in human clinical specimens. It targets a highly conserved region of the Dengue genome, allowing it to detect all four major serotypes: DENV1, DENV2, DENV3, and DENV4.

This real-time PCR assay is designed for use with serum or plasma (EDTA) samples and provides:

• A fluorescent signal in the FAM channel for Dengue RNA

• A signal in the TEXAS RED channel for the internal endogenous control (IC)

All necessary reagents—including enzymes, primers, probes, and controls—are included in the kit. The internal control plays a key role in ensuring test reliability by identifying issues such as PCR inhibition or extraction failure.

Role of Dengue Serotypes in Disease

Dengue is a mosquito-borne viral infection caused by four distinct but related viruses (DENV1–4). It affects hundreds of millions of people annually, particularly in tropical and subtropical regions.

While many infections are mild or asymptomatic, Dengue-positive patients can develop more severe manifestations such as Dengue hemorrhagic fever or Dengue shock syndrome. These are often associated with secondary infections by a different serotype due to antibody-dependent enhancement (ADE).

Accurate detection of all serotypes is critical for:

• Clinical diagnosis and risk assessment

• Treatment monitoring and relapse prevention

• Public health surveillance and outbreak control

The TRUPCR® kit offers broad serotype coverage, helping laboratories support fast and effective patient care.

About the TRUPCR® Dengue Detection Kit

This one-step real-time reverse transcription PCR kit combines RNA reverse transcription and cDNA amplification in a single tube, reducing contamination risk and streamlining workflow.

Key specifications:

• Target: Conserved region of Dengue RNA (all serotypes)

• Sample types: Human serum or EDTA plasma

• Internal Control: RNaseP or other endogenous IC

• Time to result: ~90 minutes from RNA input

• Certifications: CE-IVD

Validated across a large set of clinical samples, the assay delivers reliable, reproducible results even in early-phase infections or samples with low viral load.

Principle and Workflow

The TRUPCR® Dengue Detection Kit uses a one-step RT-PCR method. Here’s how it works:

1. RNA Extraction: Viral RNA is isolated from serum or plasma.

2. Reaction Setup: All reagents are mixed in a single tube.

3. Reverse Transcription: RNA is reverse-transcribed into complementary DNA (cDNA).

4. Real-Time PCR Amplification:

   • FAM Channel: Detects Dengue viral RNA

   • TEXAS RED Channel: Detects endogenous internal control

The internal control validates both extraction and amplification phases, ensuring that negative results are not due to sample degradation or technical issues.

Thanks to the one-tube design and hot-start technology, the risk of cross-contamination is minimized while maintaining high sensitivity and specificity.

Clinical Application

This kit is suitable for:

• Routine diagnosis in Dengue-endemic areas

• Emergency screening during outbreaks

• Early detection before symptom onset

• Therapy monitoring in hospitalized patients

• Post-treatment relapse checks in convalescent individuals

Given the public health burden of Dengue, early and accurate Dengue-targeted testing is critical. Real-time PCR assays like this one allow medical laboratories to deliver results rapidly and confidently, guiding clinical decisions and supporting infection control.

Its reliability in detecting all four serotypes also makes it an excellent tool for epidemiological studies and for monitoring vaccine responses or therapeutic trials.