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The GeneProof Aspergillus PCR Kit is a highly sensitive and accurate solution for detecting Aspergillus terreus. It targets the multicopy sequence of the Intergenic spacer ITS2/28S rDNA, and it enables specific antifungal therapy of resistant aspergillosis. The kit has a simple laboratory workflow, easily combinable with other GeneProof PCR kits, and a single tube Ready-to-Use Master Mix that eliminates the need for additional pipetting of PCR reagents. The kit is CE IVD certified and compatible with a wide range of real-time PCR devices. The Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs, eliminating possible carryover contamination, ensuring accurate and reliable results. With the GeneProof Aspergillus PCR Kit, you can detect Aspergillus terreus with ease and confidence, ensuring the best possible outcome.
Introducing the GeneProof Aspergillus PCR Kit – the ultimate solution for detecting Aspergillus terreus with high sensitivity. This kit targets the multicopy sequence of the Intergenic spacer ITS2/28S rDNA, ensuring accurate and reliable results every time.
What sets this kit apart is its ability to enable specific antifungal therapy of resistant aspergillosis, ensuring the best possible outcome for your patients. The GeneProof Aspergillus PCR Kit is also designed with a simple laboratory workflow, easily combinable with other GeneProof PCR kits in one workflow, ensuring efficient use of your laboratory resources.
The single tube Ready-to-Use Master Mix contains all components for PCR amplification, eliminating the need for additional pipetting of PCR reagents, making it easy to use and simplifying your laboratory workflow. Additionally, the Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs, eliminating possible carryover contamination, ensuring the accuracy of your results.
This CE IVD certified diagnostic test is compatible with a wide range of real-time PCR devices, making it a versatile and practical solution for your laboratory. With the GeneProof Aspergillus PCR Kit, you can detect Aspergillus terreus with ease and confidence, ensuring the best possible outcome.
| Indication | in vitro diagnostic medical device |
| Regulatory Status | CE IVD / EC Directive 98/79/EC |
| Intended User | For professional use in laboratories with trained staff |
| Technology | Real-time PCR |
| Type of Analysis | Qualitative |
| Target Sequence | ITS2/28S rDNA region |
| Analytical Specificity | Aspergillus spp. (A. fumigatus, A. flavus, A. niger, A. oryzae, A. candidus, A. foetidus, A. nidulans, A. ustus, A. versicolor, A. wentii, A. clavatus, A. niveus) (ASPspp), 100 %
Aspergillus terreus (ASPT), 100 % |
| Analytical Sensitivity (LoD with 95% probability) | 40.76 cp/ml, performed on AmpliRun® Aspergillus fumigatus DNA control (Vircell) |
| Extraction/Inhibition Control | PCR inhibition and DNA extraction efficiency control by Internal Standard (IS) |
| Validated Specimen | Whole blood, plasma, serum, BAL, sputum, CSF |
| Storage | -20 ± 5 °C |
| Validated Extraction Methods | croBEE 201A Nucleic Acid Extraction Kit GeneProof PathogenFree DNA Isolation Kit |
| Validated Instruments | croBEE Real-Time PCR System Applied Biosystems 7500 Real-Time PCR System Aria Mx Real-Time PCR System CFX96™/ Dx Real-Time PCR Detection System LightCycler® 480 LineGene 9600/9600 Plus Mic qPCR Cycler QuantStudio™ 5 Real-Time PCR System Rotor-Gene 3000/Q AMPLilab RT PCR System Bio Quant-96 Gentier 96E/96R Real-Time PCR System SLAN® Real-Time PCR System |
| Required Detection Channels | FAM (ASPspp), Cy5 (ASPT), HEX/JOE/VIC (IS) |
| External Quality Assessment | Regularly tested in QCMD External Quality Assessment panels – results at www.geneproof.com |
Instructions for Use
Declaration of Conformity
MSDS
Publications
Manual
PCR Templates
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