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MTHFR II Polymorphism Kit

Short description

Unleash Unmatched Accuracy with the MTHFR II Polymorphism Kit

Designed for molecular biology professionals, the MTHFR II Polymorphism Kit offers a reliable and accurate method for detecting important variants in the MTHFR gene. Using a validated real-time PCR assay, the kit provides exceptional precision and includes a positive control for dependable results. It’s optimized for efficiency, requiring just 50ng of genomic DNA, and is ISO-certified for quality and sustainability.

Elevate your diagnostic capabilities while contributing to a more sustainable future.

Product highlights

  • Real-Time PCR
  • Precision Allele Detection
  • Fluorescent Hydrolysis Probes
  • Detection of c.1298A>C


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MTHFR II Polymorphism Kit



Instructions for Use

MSDS MTHFR II Polymorphism Kit


For any missing information or if you require additional details, please do not hesitate to contact us. 

Specifications of the MTHFR II Polymorphism Kit

Unlock Genetic Insights with the MTHFR II Polymorphism Kit

The MTHFR II Polymorphism Kit is a cutting-edge solution for professionals in molecular biology, offering an accurate and reliable method for detecting key variants in the MTHFR gene. This gene plays a vital role in folate metabolism, which is essential for numerous bodily functions, including the synthesis of the amino acid methionine, a crucial building block for proteins.

The MTHFR gene, located on chromosomal region 1p36.22, encodes the enzyme methylenetetrahydrofolate reductase, critical for the processing of vitamin B9 or folate. Variants such as the c.1298A>C polymorphism can result in reduced enzymatic activity. This has been linked to an increased risk of developmental defects including anencephaly and spina bifida, as well as other conditions like homocystinuria and vascular issues.

The kit has undergone rigorous validation using samples analyzed by Health in Code S.L.’s medical genetics service. It’s certified according to the standard UNE-EN ISO 13485:2018 by the Spanish Agency for Medicinal Products and Medical Devices (AEMPS).

Features That Set the kit Apart

Precision and Specificity: Utilizes a validated real-time PCR assay that combines specialized oligonucleotides and fluorescent hydrolysis probes for the qualitative detection of the pathogenic allele c.1298A>C and the normal allele of the MTHFR gene.
Complete Control: Includes a positive control, a v/v mixture of synthetic DNA, which helps in establishing the reliability of the PCR system and aids in qualitative analysis.
Efficiency: Specifically designed to detect expected genotypes in the MTHFR gene, it requires only 50ng of genomic DNA from peripheral blood for each assay.
Compatibility: Validated using the same real-time PCR program as products related to coagulation process alterations, allowing for simultaneous analysis.
Compliance: Meets the stringent quality standards as defined by ISO 13485 and ISO 14001 regarding the materials used in its manufacturing process.

In alignment with the commitment to sustainability, the kit’s packaging material is made in compliance with ISO 14001, using 30-100% recycled material.

The Bottom Line
The MTHFR II Polymorphism Kit is not just a product; it’s a comprehensive solution for healthcare providers seeking reliable, efficient, and environmentally responsible options for genetic analysis. By employing this kit, you are not only ensuring precise diagnostic results but also contributing to a greener future.

Unlock the gold standard in genetic health screening with the MTHFR II Polymorphism Kit.

Components of the MTHFR II Polymorphism Kit

The kit contians the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:

  • MTHFRII Master Mix: Specific oligonucleotides, labelled probes and water reagents for the
    PCR reactions.
  • General Master Mix: Conventional PCR enzyme containing 10-30% glycerol, <0.1 sodium
    azide and buffers.
  • Positive Control: Heterozygous positive control for the c.1298A>C (p. Glu433Ala) mutation
    in MTHFR gene.

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