Quality Controls & Reference Standards > FFPE

PIK3CA-E542K 50% AF FFPE Reference Standard curl

Name: PIK3CA-E542K 50% AF FFPE Reference Standard curl - Goffin Molecular Technologies
Availability: InStock

Quality Controls & Reference Standards > FFPE

PIK3CA-E542K 50% AF FFPE Reference Standard curl

Product highlights

Validates DNA/RNA extraction protocols with the reliable PIK3CA-E542K 50% AF FFPE Reference Standard curl.
Enables quality control for sequencing and PCR processes.
Supports sensitivity calibration for instruments and methods.
Perfect for regular accuracy checks in research settings.

PIK3CA-E542K 50% AF FFPE Reference Standard curl

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About PIK3CA-E542K 50% AF FFPE Reference Standard curl

The PIK3CA-E542K 50% AF FFPE Reference Standard curl is a formalin-fixed paraffin-embedded (FFPE) reference material created to simulate patient samples, enhancing reliability in clinical genomics and diagnostic workflows. This human cell line-derived standard is perfect for use in diagnostic labs and R&D settings, enabling full workflow control for FFPE-based assays and extraction kit development.

With high-quality mutations across 57 genes, including PIK3CA-E542K at 50% allelic frequency, this reference curl supports several critical applications:

Validates and refines DNA/RNA extraction protocols.
Provides quality control in sequencing and PCR workflows, including WGS and WES.
Calibrates and tests the sensitivity limits of laboratory instruments and protocols.
Supports performance monitoring and routine accuracy checks in research applications.

Key specifications include a minimum yield of 400 ng for both DNA and RNA, 10 µm thickness per curl, and a 24-month stability when stored at 2-8°C. This PIK3CA-E542K 50% AF FFPE Reference Standard curl is an essential tool for clinical genomics and molecular diagnostics, ensuring reliable, reproducible results.

Specifications of the PIK3CA-E542K 50% AF FFPE Reference Standard curl

The PIK3CA-E542K 50% AF FFPE Reference Standard curl is a human cell line-derived formalin-fixed paraffin-embedded (FFPE) reference material designed to closely replicate patient samples in clinical and research applications. This reference standard is particularly valuable as a full workflow control in diagnostic labs or R&D settings, supporting the validation and development of FFPE-based diagnostic assays or extraction kits. Details on additional gene mutation statuses are available on the product page.

Ideal for enhancing accuracy in clinical genomics workflows, the PIK3CA-E542K 50% AF FFPE Reference Standard curl provides essential quality control for a variety of laboratory processes, including:

Validation and protocol development: Ideal for refining extraction protocols to ensure reliable DNA/RNA isolation.
Sequencing protocol control: Optimize whole genome sequencing (WGS), whole exome sequencing (WES), amplicon sequencing, and PCR workflows.
Detection limit establishment: Determine the sensitivity and detection threshold of various methods.
Instrument calibration: Develop and calibrate DNA processing workflows, such as DNA fragmentation via acoustic shearing, enzymatic digestion, or sonication.
NGS pipeline performance analysis: Evaluate and verify the performance and consistency of next-generation sequencing (NGS) processes.
Routine monitoring: Ensure the accuracy and repeatability of results in research applications (Research Use Only).

Product Overview

Format: FFPE (Formalin Fixed Paraffin Embedded) standard
Unit size: 1 curl per vial
Expected DNA yield: At least 400 ng, using the Qiagen AllPrep DNA/RNA FFPE kit
Expected RNA yield: At least 400 ng, using the Qiagen AllPrep DNA/RNA FFPE kit
Thickness: Each curl is precisely 10 µm in thickness

Mutations and Testing

This reference standard contains 62 cancer-relevant mutations across 57 genes, with a full mutation list available under the “Additional Genes Covered” tab on the product page. Specifically tested mutations include PIK3CA-H1047R(HGVS: c.3140A>G) and PIK3CA-D350N (HGVS: c.1048G>A), verified with digital droplet PCR (ddPCR) for precise allelic frequency determination. Additionally, this reference curl is representative of a 50% allelic frequency for the PIK3CA-E542K mutation, ensuring consistency in experimental results.

Key Attributes:

Allelic frequency for PIK3CA mutations: PIK3CA-E542K 50%, validated via ddPCR.
Storage conditions: Store at 2-8°C.
Shelf life: Stable for 24 months from manufacturing date.

The PIK3CA-E542K 50% AF FFPE Reference Standard curl delivers robust performance for clinical genomics and diagnostic assay development, providing essential support in refining FFPE-based workflows, calibrating instruments, and maintaining quality control across multiple genetic analysis platforms.