LCT 13910C>T RealFast ™ RT-PCR Assays

Lactose introlerance LCT 13910CT PCRLCT 13910CT PCR

The ViennaLab LCT 13910C>T RealFast RealFast assays detect the most commen polymorphism causing lactase non persistence. LCT 13910CT PCR

Lactose and Fructose are among the most common sugars in hum diet, but not all people have the necessary enzymes in sufficient quantities to completely digest them. Mutations in the genes for metabolic enzymes or transporters can couse hereditary sugar intolerance. People who sugger from this intolerance may experience severe discomfort when eating lactose and fructose. Commen Symptoms inculde abdominal bloating and pain, vomiting, diarrhea, nausea, flatulence and hypoglycemia.

LCT 13910C>T RealFast ™ RT-PCR Assays technology

The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes. The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore. During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye. This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.

For the StripAssay variants of this test click here

LCT 13910C>T RealFast

LCT 13910C>T RealFast™7-150100 testsDetects the most common polymorphism causing lactase non-persistence