BIOSYNEX AMPLIQUICK® Protozoans: Real-Time PCR Detection of Intestinal Parasites

Accurate, rapid, and multiplex molecular diagnostics for intestinal protozoans

Intestinal parasite infections remain a major global public health concern, affecting millions across both developing and industrialized countries. These infections, caused by intestinal protozoans such as Giardia intestinalis and Entamoeba histolytica, can lead to serious gastrointestinal symptoms and complications, especially in immunocompromised patients.

Molecular detection of 10 intestinal Protozoans

The BIOSYNEX AMPLIQUICK® Protozoans kit is an advanced real-time PCR diagnostic test designed for the qualitative detection of ten intestinal protozoans directly from DNA extracted from stool samples.

This ready-to-use, highly sensitive assay targets the following parasites:

• Cryptosporidium spp

• Dientamoeba fragilis

• Enterocytozoon bieneusi

• Encephalitozoon spp

• Entamoeba histolytica

• Entamoeba dispar

• Blastocystis spp

• Giardia intestinalis

• Cystoisospora belli

• Cyclospora cayetanensis

Convenient format, streamlined workflow

The BIOSYNEX AMPLIQUICK® Protozoans kit is an in vitro diagnostic test that utilizes real-time PCR technology. This kit comes in three different versions. Each kit consists of microplates with 12 strips of 8 wells, where the first 5 wells of each strip are pre-filled with master mixes and the last 3 are empty. This allows for testing of one patient sample per strip. The master mixes are ready to use and contain dNTPs, MgCl2, fluorescent primers and probes, Taq polymerase, and reaction buffer. The test kit is presented in the form of 96-well plates that can be easily divided into strips of 8 wells each for convenience. With this kit, lab technicians can quickly and accurately detect the presence of ten intestinal protozoans from DNA extracted from stool samples.

Complementary kit for Helminth detection

A second kit, BIOSYNEX AMPLIQUICK® Helminths (Ref 3150066), is also available to detect ten intestinal helminths from the same DNA extracts. Protozoan infections, caused by single-celled parasites such as Entamoeba histolytica or Giardia lamblia, can produce severe symptoms and even death, particularly in immunocompromised patients. This kit provides higher sensitivity and specificity than conventional microscopy, the ability to target multiple parasites, faster results, and reduced turnaround time, making it an essential tool for laboratory staff for in vitro molecular diagnostics.