HLA-B Gene Kit – Real-Time PCR Detection of HLA-B27 Allele

The HLA-B Gene Kit is a qualitative real-time PCR assay specifically developed for the detection of the HLA-B27 allele, a key genetic marker associated with autoimmune inflammatory diseases such as Ankylosing Spondylitis (AS) and other spondyloarthropathies. This high-performance diagnostic tool is designed for use in molecular biology and clinical laboratories that require accurate, rapid, and reliable HLA-B27 genotyping.

Precision Diagnostics for HLA-B27 Detection

The HLA-B27 allele, located within the major histocompatibility complex (MHC) on chromosome 6p21, plays a critical role in the immune system’s ability to recognize and respond to pathogens. Variants in this gene are closely associated with the development of autoimmune conditions affecting the spine and joints. Additionally, HLA-B27 has been linked to improved immune outcomes in certain viral infections, such as HIV and hepatitis C.

The  Kit utilizes a real-time PCR approach targeting exon 2 of the HLA-B gene. It is capable of amplifying and detecting various subtypes of the B27 allele with high specificity and sensitivity. This assay is intended for screening and genetic investigation of autoimmune diseases—not for organ transplant compatibility testing.

Key Features

Accurate and Sensitive Detection

• Targets specific polymorphisms in exon 2 of the HLA-B gene

• Enables precise identification of HLA-B27 subtypes using multiplex PCR

Built-In Quality Control

• Includes endogenous β-globin gene as an internal control to verify DNA integrity and test performance

Validated for Clinical Use

• Validated using reference materials from respected institutions including the Coriell Institute and C.H.U. Insular Materno-Infantil

• Demonstrated accuracy and reproducibility in routine diagnostics

Low Sample Input, High Efficiency

• Requires only 50 ng of genomic DNA

• Compatible with DNA extracted from peripheral blood or saliva

Intended Use

The HLA-B Gene Kit is intended for in vitro diagnostic use in molecular laboratories for the detection of the HLA-B27 allele. It supports the early diagnosis and risk assessment of autoimmune inflammatory diseases, especially in patients with symptoms related to spinal and joint disorders.

Reliable

With its advanced molecular design, built-in quality controls, and proven clinical relevance, the HLA-B Gene Kit offers a robust solution for genetic screening and autoimmune disease diagnostics. It empowers laboratories with the tools needed for precision medicine and improved patient care.