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Factor II kit

Short description

Unlock Coagulation Genetics with Factor II PCR Kit

The Factor II PCR Kit is a groundbreaking diagnostic tool designed for molecular biologists focusing on the F2 gene’s role in blood coagulation. Utilizing a validated real-time PCR assay with advanced fluorescent hydrolysis probes, the kit offers unparalleled accuracy and specificity. Tailored for professionals, it requires just 50 ng of genomic DNA for each test.

Embark on a new frontier of personalized medicine and genetic research.

Product highlights

  • Real-Time PCR
  • F2 Gene Focus
  • Dual Probes
  • Target Multiplexing


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Factor II kit



Instructions for Use

MSDSHLA Factor II kit


For any missing information or if you require additional details, please do not hesitate to contact us. 

Specifications of the Factor II kit

Factor II PCR Kit – The Benchmark in Coagulation Genetics Analysis

Within the multifaceted landscape of molecular biology and medical genetics lies the opportunity to scrutinize genetic variants with profound implications. The Factor II PCR Kit offers an unparalleled instrument for specialists in the molecular biology domain who aim to explore the F2 gene, pivotal in blood coagulation mechanisms.

Central to the Factor II PCR Kit is its validated real-time PCR diagnostic assay. This state-of-the-art system employs a meticulous blend of oligonucleotides and fluorescent hydrolysis probes. Specifically, it targets the detection of the pathogenic allele c.*97G>A and the normal allele in the 3′ UTR region of the F2 gene (prothrombin). The inclusion of genomic DNA heterozygous for the target mutation as a positive control adds an extra layer of reliability and rigor.

Key Features Adapted for Precision

Genomic Focus: The assay specializes in targeting the F2 gene, providing in-depth and accurate insights. A mere 50 ng of genomic DNA (gDNA) is needed for each assay.

Reliable Assay: Validation from reference DNA samples and synthetic DNA ensure the assay’s unparalleled robustness.

Advanced Probes: Utilizes fluorescent hydrolysis probes for precise allele identification.

User Requirements: Intended solely for in vitro diagnostics and for professionals in molecular biology.

Equipment Compatibility: The kit is tailored for real-time PCR systems with standard fluorescence channels.

Sample Versatility: Genomic DNA extracted from peripheral blood is the ideal sample, retaining high sensitivity and specificity.

The New Era with Factor II PCR Kit

Navigating the complexities of molecular biology requires tools of extraordinary precision and reliability. The Factor II PCR Kit stands as a colossal leap forward in coagulation genetics analysis. Crafted with extreme accuracy and leveraging avant-garde technology, this diagnostic kit presents molecular biologists with the keys to uncharted realms in blood coagulation research.

For those eager to lead in the sphere of personalized medicine and genetic diagnostics, the next echelon of scientific exploration is within your grasp. Embark on this unprecedented journey in molecular biology; your scientific odyssey begins here.

Components of the Factor II kit

The kit contents the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:

  • Factor II Master Mix: Specific oligonucleotides, labelled probes and water reagents for the
    PCR reactions.
  • General Master Mix: Conventional PCR enzyme containing 10-30% glycerol and buffers.
  • Positive Control: Heterozygous positive control for the c.20210G>A mutation in F2 gene.

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