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Huntington Disease Kit

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Introducing the Imegen® Huntington Disease Kit for In Vitro Diagnostic Use:

The Imegen® Huntington Disease Kit offers semi-quantitative analysis of CAG expansion repetitions within the HTT gene through two PCR assays (HTT-C and HTT-L). It covers an amplification range of 100-500 base pairs and includes a positive control with normal alleles for all kit assays. Designed and validated alongside related products for neurodegenerative disease analysis, this kit meets ISO 13485 and ISO 14001 quality standards for materials used in its manufacturing process.

Product highlights

  • CAG Expansion Analysis
  • Semi-quantitative PCR
  • Positive Control Included
  • In Vitro Diagnostic
  • Compatible Thermal Cyclers
  • ISO Quality Standards

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Huntington Disease Kit

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Imegen® Huntington Disease Kit

 

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Specifications of the Huntington Disease Kit

Introducing Imegen® Huntington Disease Kit for In Vitro Diagnostic Use:

Product Overview: Imegen® Huntington Disease Kit allows semi-quantitative analysis of CAG expansion in the HTT gene via HTT-C and HTT-L PCR assays. It covers a 100-500 bp amplification range, featuring a positive control with normal alleles. Validated with related neurodegenerative disease products, it complies with ISO 13485 and ISO 14001 quality standards.

Key Features:

  • Semi-Quantitative CAG Analysis
  • Dual PCR Assays (HTT-C and HTT-L)
  • 100-500 bp Amplification
  • Included Positive Control
  • Compatible with Neurodegenerative Disease Assays
  • In Vitro Diagnostic
  • 50 ng DNA per Reaction
  • Peripheral Blood Samples
  • 2 Reactions per Sample
  • Targets: CAG and poly-CCG
  • Quick Setup (45 mins)
  • PCR Program (2h 15 mins)
  • Compatible with Standard Cyclers
  • 6-FAM and NEDTM Sequencing

About Huntington’s Disease: Huntington’s Disease is an inherited neurodegenerative disorder causing progressive brain neuron degeneration, impacting motor skills, cognition, and mental health. Diagnosis hinges on CAG triplet repeat analysis, with <26 repeats as normal, 27-35 intermediate, and >36 as pathological.

Imegen® Huntington Kit offers precise CAG expansion analysis with HTT-C (short-range) and HTT-L (long-range) PCR, covering polymorphic poly-CCG fragments. Sequencing via 6-FAM and NEDTM-labeled PCR products separates amplicons for size analysis.

References:

  • Potter NT, Spector EB, Prior TW. Technical standards and guidelines for Huntington disease testing. Genet Med. 2004 Jan-Feb;6(1):61-5.
  • Losekoot M et al. EMQN/CMGS best practice guidelines for the molecular genetic testing of Huntington disease. Eur J Hum Genet. 2013 May;21(5):480-6.

Imegen® Huntington Kit is designed for in vitro diagnostic use, exclusively for molecular biology professionals.

 

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Introducing Imegen® Huntington Disease Kit for In Vitro Diagnostic Use:

Product Overview: Imegen® Huntington Disease Kit enables semi-quantitative analysis of CAG expansion in the HTT gene through HTT-C and HTT-L PCR assays, covering a 100-500 bp amplification range. It includes a positive control with normal alleles and complies with ISO 13485 and ISO 14001 quality standards.

Key Features:

  • Semi-Quantitative CAG Analysis
  • Dual PCR Assays (HTT-C and HTT-L)
  • Amplification Range: 100-500 bp
  • Included Positive Control
  • Compatible with Neurodegenerative Disease Assays
  • In Vitro Diagnostic
  • DNA Requirement: 50 ng per Reaction
  • Sample Type: Peripheral Blood
  • 2 Reactions per Sample
  • Targets: CAG and poly-CCG
  • Quick Setup (45 mins)
  • PCR Program Duration: 2h 15 mins
  • Compatible with Standard Cyclers
  • Sequencing with 6-Carboxyfluorescein (6-FAM) and NEDTM

About Huntington’s Disease: Huntington’s Disease is an inherited neurodegenerative disorder causing progressive brain neuron degeneration, impacting motor skills, cognition, and mental health. Diagnosis hinges on CAG triplet repeat analysis, with <26 repeats as normal, 27-35 intermediate, and >36 as pathological.

Imegen® Huntington Kit offers precise CAG expansion analysis with HTT-C (short-range) and HTT-L (long-range) PCR, covering polymorphic poly-CCG fragments. Sequencing via 6-FAM and NEDTM-labeled PCR products separates amplicons for size analysis.

Imegen® Huntington Kit is designed for in vitro diagnostic use, exclusively for molecular biology professional

Components of the Huntington Disease Kit

Huntington Master Mix, General Master Mix IV, HTT-L Master Mix, HTT-C Master Mix and Positive Control

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