MolTaq 16S/18S is a highly purified recombinant thermostable DNA polymerase enzyme that is isolated from Thermus aquaticus. This enzyme has a high specific activity and a high processivity, which enables it to amplify DNA fragments up to 5 kb in length. The enzyme has a proofreading activity, which makes it highly accurate in the replication of DNA. Additionally, MolTaq 16S/18S has a low error rate, which means that it is highly reliable in PCR experiments.
There are several advantages of using MolTaq 16S/18S in PCR experiments. Firstly, the enzyme has a high sensitivity, which means that it can amplify low amounts of DNA. This is particularly useful in experiments where the starting material is limited. Secondly, MolTaq 16S/18S has a high yield, which means that it can generate a large amount of amplified DNA in a short period of time. This is particularly useful in experiments where a large amount of DNA is required, such as sequencing or cloning.
Another advantage of MolTaq 16S/18S is its ability to amplify DNA from a variety of sources. The enzyme can amplify DNA from different organisms, tissues, and fluids, making it a versatile tool for molecular biology experiments. Additionally, MolTaq 16S/18S is compatible with a variety of PCR buffers and conditions, which makes it easy to use in different experimental setups.
MolTaq 16S/18S has several applications in molecular biology research. It is commonly used in PCR experiments for the amplification of DNA fragments for sequencing or cloning. The enzyme is also used in the detection of microbial pathogens in clinical and environmental samples. MolTaq 16S/18S is particularly useful in the amplification of 16S and 18S rRNA genes, which are commonly used as genetic markers for microbial identification and taxonomy.