The UTI Panel by TRUPCR® is a multiplex real-time PCR assay for the qualitative detection and differentiation of urinary tract infections caused by 14 bacterial and fungal pathogens. The UTI Panel is designed to deliver results in under 60 minutes, making it one of the fastest molecular diagnostic tools for UTI-positive identification. Using five tubes and four fluorescent channels, this assay enables simultaneous detection of common and rare uropathogens, including E. coli, Klebsiella, Proteus, Pseudomonas, Candida, and more. The assay integrates an internal control and UNG treatment to minimize contamination risk and maximize accuracy. Its room-temperature setup and compatibility with standard qPCR systems make it an ideal solution for clinical and microbiology labs.
The TRUPCR® UTI Panel is a multiplexed real-time PCR assay targeting 14 clinically relevant uropathogens. These include both Gram-positive and Gram-negative bacteria, as well as a fungal species. The panel includes:
Enterococcus faecalis, Klebsiella oxytoca, Escherichia coli, Acinetobacter baumannii, Streptococcus agalactiae, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecium, Proteus mirabilis, Streptococcus pneumoniae, Candida albicans, Staphylococcus saprophyticus, Morganella morganii, and Pseudomonas aeruginosa.
Each tube in the panel targets 2–3 pathogens using pathogen-specific probes labeled with FAM, Texas Red, and Cy5 dyes. The setup enables detection of co-infections in a single workflow.
Together, these organisms cover over 95% of known UTI etiologies in diverse patient populations.
The TRUPCR® UTI Panel is a ready-to-use multiplex PCR kit designed for rapid, accurate detection of urinary tract infections. With a total run time under 60 minutes, the assay offers one of the fastest time-to-result for UTI diagnostics.
It is validated on over 500 clinical samples and is compatible with commonly used real-time PCR platforms. The inclusion of UNG (uracil-N-glycosylase) in the master mix prevents carryover contamination from previous PCR runs.
A built-in endogenous control in every reaction tube monitors PCR inhibition, sample integrity, and extraction efficiency.
The UTI Panel employs TaqMan hydrolysis probe chemistry and consists of the following steps:
All reagents are optimized for rapid setup and minimal hands-on time.
The TRUPCR® UTI Panel is ideal for:
Its ability to detect co-infections, including rare fungal and resistant strains, makes it especially valuable for improving antimicrobial stewardship and guiding treatment decisions in real-time.
Suggested Image ALT Text: Multiplex UTI Panel kit detecting E. coli, Klebsiella, Candida, and other UTI pathogens
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